Respiratory Metabolism Of Queen Capped Broods Of Apis Cerana Ceranaand And Apis Meltifera Ligustica

In this experiment, the change rule of oxygen consumption between queen capped broodsof Apis cerana cerana and Apis mellifera ligustica was studied at34.5℃. Furthermore, weinvestigated the correlation among fresh weight, egg weight, fecundity of queens and oxygenconsumption on the first, fifth and seventh day of capped brood at34.5℃. The results were asfollows:(1) By the study of oxygen consumption between Apis cerana cerana capped queen broodand Apis mellifera ligustica capped queen brood, the oxygen consumption of the one-daycapped queen brood was found the highest. And with the development of capped queen cells,the oxygen consumption was decrease firstly and then increased, presenting “U” shape.Between Apis cerana cerana and Apis mellifera ligustica capped queen cells, after capping1and2days, the comsumption of Apis mellifera Ligustica was significantly higher than that ofApis cerana cerana; Apis mellifera ligustica is higher than Apis cerana cerana. Overall, theoxygen consumption of Apis cerana cerana is more closely to U-shaped curve than Apismellifera ligustica.(2) The weight change of capped queen cell during metabolic process was studied on Apiscerana cerana and Apis mellifera ligustica in the whole capped time. The result showed that thetrend of one-day reduced value was same roughly in both honeybees. The value decreased withthe growth and development of capped queen cell. However, the reduced weight of cappedseven days of Apis cerana cerana after capped seven days was0.0543g, while Apis melliferaLigustica was0.0716g. An interesting phenomenon during studying the queen cells dailyweightless caused by metabolism is that the weight loss of queen capped brood is apparentlydifferent between Apis cerana cerana and Apis mellifera ligustica. When capped two days ofApis cerana cerana, the weight loss of Apis cerana reached a peak of0.012g, after three daysquickly decline to lowest value of0.0083g, after four days it rose to the second peak of0.097g,then slowly decline; for Apis mellifera ligustica, the weight loss upto maximum was one daylater than Apis cerana cerana, three days the weight reduced0.0168g which was the highest. Itappeared two valleys, the first value was0.0118g, the second is0.0085g. Because the last ageof queen larvae need molting and excretio, the loss of moisture was upto the maximum,Therefore, we can infer that the peak of reduced values should be occurred in molting andexcretion period of queen capped brood. So in other words, the time of molting and excretion ofqueen capped brood of Apis mellifera ligustica was one day later than that of Apis ceranacerana. In the meanwhile, from the appearance of the valley value0.0085g at six days after sealing, we can see that the queen pupal of Apis mellifera ligustica started eclosion metabolismagain after a short rest, but this phenomenon did not occur in Apis cerana cerana.(3) By means of the research about the relationship among the oxygen consumption, birthweight, egg weight and the total amounts of eggs when respectively capped for the first day,fifth day, seventh day of Apis cerana cerana and Apis mellifera ligustica, except egg weightthey had the significant positive correlation when capped first day and seventh day(P<0.05). Butthere is no significant correlation at the fifth day(P>0.05). In this experiment,the relationshipbetween oxygen consumption and egg weight is unable to conclude.The metabolic activity wasweak when capped the fifth day, then it may not reflect its characteristic, while the metabolicactivity of capped one and seven days was intense, therefore the oxygen consumption at the firstday or seventh day can be a standard to evaluate the queens quality.The metabolic activity wasweak when capped the fifth day, then it may not reflect its characteristic, while the metabolicactivity of capped one and seven days was intense, therefore the oxygen consumption at the firstday or seventh day can be a standard to evaluate the queens quality.