| Camptotheca acuminate is a deciduous tree belonged to Nyssaceae. It is one of therare tree species, and mainly distributed in the basin of Yangtze River in China. Thisspecies is not only a quality green tree and construction material, but also has highlymedicinal value for the anti-cancer function of its second metabolite camptothecin. Inrecent years, as the increasing demands for anti-cancer drugs, the study of Camptothecaacuminate is more and more in-depth. Seeds are the beginning of plants’ life, and seedgermination affects the growth and development of plants. Although many progresseshave been made in the seed germination research, its regulation mechanism have notbeen clarified yet. In the research of seed germination, proteomics technology is criticalfor observing the dynamic changes of proteins during seed germination, and it lays thefoundation for in-depth understanding the regulation mechanism of seed germination atthe molecular level.We investigated the morphological characteristics of mature seed, imbibed seedand seed germination of Camptotheca acuminate. We separated proteins and analysedthe differentially expression of proteins by using proteomic technology and statisticalmethod. Spots with more than a1.5-fold change were considered to be differentiallyexpressed spots. In this study, we obtained173differentially expressed spots. Of these,94spots up-regulated and2spots down-regulated when imbibed seed compared withmature seed,132spots up-regulated and15spots down-regulated when germination ofseed compared with mature seed. During the germination of Camptotheca acuminateseed, the expression abundance of many proteins presented significant changes. Theseresults imply that Camptotheca acuminate seed completes various kinds ofphysiological and biochemical reactions in germination by regulating the expressionabundance of metabolic-related proteins. |
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