| The purpose of this topic is to test strains straw actually using the value increase as the theory basis, using microbial transformation renew resources, conservate resources, to solve the environmental pollution, the use of Trichoderma mildew and white-rot fungus cellulose degradation of the function can increase the protein content in the straw stalk, and improve the utilization value. Choosing the best production condition, so as to promote in the actual production process, the test is divided into three parts:First of all, Identified the best conditions of Trichoderma fermentation of straw in room temperature:The results showed that the optimum fermentation conditions in room temperature were as follows:nitrogen source was urea, additive amount3%and inoculum concentration2%,the ratio between straw and wheat bran was7:3,additive amount of nutrient solution150%,initial pH value3.0,fermentation time96h,CMC and FPA were the most active.The second, On the basis of a single fermentation conditions, the application of multi-strain mixed fermentation of rice straw as substrate culture, Mixed cultures interact to make up for the shortcomings of a single strain effect, test the method of orthogonal experimental design,Identified Trichoderma, white rot fungus and yeast mixed fermentation medium, and then screened the best culture conditions of the mixed bacteria through a single factor. The results showed that: Strain Compatibility tests proved that Trichoderma Koningi and Yeast group, White-rot fungi and Yeasts group can be compatibly symbiotic, while there was a certain antagonistic effect between Trichoderma Koningi and White-rot fungi group. But through screening the optimal conditions of of mixed bacteria, it can reduce the antagonist and get the best results. The optimal medium composition of mixed fermented rice straw was:straw to wheat bran6to4,2%urea and70%nutrient solution. The inoculum concentration of mixed bacteria was2mL, inoculation ratio (Trichoderma Koningi:white-rot fungi:yeast)1:2:1, cultured for6days. In the fermentation end product, the crude protein content increased from3.49%to16.59%, while the crude fiber content decreased from44.56%to23.17%. Finally, The test selected4close to the body condition, similar age of Yanbian cattle, The experimental focus on microbial fermentation of rice straw and straw degradation in rumen, at about0,6,12,24,36,48,72h determinate dry matter (DM), protein (Pr), neutral detergent fiber (NDF) and acid detergent fiber (ADF), calculate the rate of corresponding nutrient degradation. The results showed that:microbial fermentation of rice straw about6h-72h, rumen nutrient degradation rate had significant difference (P<0.05) than ordinary straw, at72h, test group dry matter degradation rate is about56.08%,compared to the control group increased68.47%; on average protein degradation rate is48.44%, compared to the control group increased36.30%; at72h microbial fermentation to grass neutral cellulose degradation rate is the highest, repeated an average of53.22%, higher than ordinary rice straw degradation rate increased by25%;at72h, microbial fermentation of rice straw acid detergent fiber is achieved on average39.47%,15.49%higher than ordinary rice straw. Two repeated groups differences were not significant, indicated that the test by external environment influence is simple, to ensure that the test results are accurate and practical. |
