| With the continuous growth of the national economy, people’s food quality standards graduallyimprove. In recent years as Sudan red in food adds the panic, people pay more and more attention to thiskind of synthetic dyes non edible food additive which is harmful to the human body. Therefore, thestudy of toxicology and molecular biology principle method, used to study the toxic substances onanimal DNA injury severity, monitoring food toxic substance in action, has very important theoreticaland practical significance for the current formulation of additives in food safety in use indicator.This study based on toxicological evaluation of exogenous substances toxic effects, is divided intofive parts: acute toxicity test, physiological toxicity test, genetic toxicity test （micronucleus test）,embryonic and larval toxicity test, DNA injury: a RAPD analysis. We preliminary discussed the toxicityand molecular biological effects of Sudan red IV （Sudan IV） on two kinds of Loach （Misgurnusanguillicaudatus,）. On the basis of the toxicology and molecular biology experimental results on SudanIV （Sudan IV） toxicity, make scientific assessment, providing digital basis for reasonable formulationof food in Sudan IV （Sudan IV） use safety indicators, in order to serve our country food safetyproduction better.Through acute toxicity test, the Sudan red IV （Sudan IV） on Loach24h median lethal concentration（median lethal concentration, LC50）2.125g.L-1,48h median lethal concentration （median lethalconcentration, LC50）1.925g.L-1, safe concentration （safe concentration, Sc）0.474g.L-1; Sudan IV（Sudan IV） on the24h median lethal concentration （median lethal concentration, LC50）2.225g.L-1,48h median lethal concentration （median lethal concentration, LC50）2.025g.L-1, safe concentration（safe concentration, Sc）0.503g.L-1.Based on the results of acute toxicity test, the genetic toxicity test （of micronuclei and nuclearanomalies test）. With blood cell micronucleus rate as an index, designed each test group concentration.Sudan IV （Sudan IV） on Loach exposure concentrations were A (0.025g.L-1), B (0.050g.L-1), C(0.100g.L-1), D (0.200g.L-1), E (0.400g.L-1),0（control group）, a total of6groups; Sudan IV （Sudan IV） on Loach exposure concentrations were A (0.030g.L-1), B (0.060g.L-1), C (0.120g.L-1),D (0.240g.L-1), E (0.480g.L-1),0（control group）, divided into6groups. Processing time in exposureafter1,2,3,4, and5days respectively with a material, observation, calculation. The results showed,in two species of loaches erythrocyte micronucleus and abnormal nucleus and Sudan IV concentrationgenerally positively correlated, but also have a certain time effect.According to the genetic toxicity test do the physiological toxicity test, with aminotransferase （GPT）,aspartate aminotransferase （GOT） activity of the two kinds of Loach Liver alanine as indicator,.Experimental data shows the liver GOT and GPT activity increased with the prolongation of time, thenfall, however, on the last day, it began to rise again, descripting loach liver has the detoxificationfunction itself. As time goes on, the GOT and GPT activity will gradually periodicly changes. Theinhibition degree of the enzyme varies from one kind of loach to another.In order to further explore the Sudan red IV （Sudan IV） in two species of loaches in the level of DNAdamage, screening8primers from50random primers, performed a RAPD （random amplifiedpolymorphic DNA） molecular markers on Loach, analysising the DNA damage of the two kinds ofloaches before and after the exposure. The analysis results show that, the Sudan red IV （Sudan IV） canto a different degree induce the addtion, deletion of the DNA fragment of the two species of loaches,and alteration of band brightness, obviously shown in the level of DNA damage effect.At the embryo toxicity test, with two kinds of loach embryo hatching rate and abnormal rate asindexes, studied the toxicity effec of the Sudan red IV （Sudan IV） on the embryo of the two kinds ofloaches. When the loaches were in the embryo gastrulation period, they were exposed to medium forcultivation, experimental results show that, Sudan IV （Sudan IV） to different degrees can reduce twokinds of loaches embryo hatching rate, increase deformity rate, leading to teratogenic, lethal effecton the embryonic development level to some extent. The degree of sensitivity varies from one kind ofloach embryo to another. |
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