Gene Clone Related To Polyketides In Monascus

Red yeast rice as Chinese traditional food are widely used in China.With the development of modern biotechnology, a number of functional components in red yeast rice has been paid more attention. Polyketides are important secondary metabolites produced by Monascus during secondary metabolism. Polyketides expressed by Monascus15-1saved in the laboratory were determined, Monascus polyketide synthase genes were carried out a preliminary exploration. The main conclusions are as follows:By analysis of morphological observation, Monascus15-1belongs to M.araneosus. By measuring the Color value of Monascus15-1cultured at different times in YES medium and the content of citrinin. Monascus-pigment and citrinin expressed by Monascus15-1fluctuated with the culture time. RNA extracted from mycelium when polyketide synthase active in Monascus15-1is suitable for polyketide synthase gene.Taking Monascus15-1as a template, two degenerate primers were designed according to LC series of primers to clone a gene qzcOl composed of388bp nucleotides. By BLAST sequence alignment through the Genbank database, qzcOI gene which shares76%similarity with lovastatin related gene in Aspergillus terreus was speculated to be related to Monacolin K’s synthesis.Three pairs of nested primers were designed by Genome walking to clone the upstream and downstream sequences of qzcOl genes. Electrophoretic analysis showed700bp.300bp and900bp gene for about respectively and speculated that they were3"and5" flanking sequence of qzcO1gene...