| Cabbage(Brassica oleracea L. var. capitata L.) is a bulb variety formed by apical bud and axillary bud of Cruciferous crops, cultivated word-widey, and it is one of the main vegetable crops in West and China. Now, the growth area of Cabbage is about 100, 0000 hectare every year in China. According to the imperfection statistics, the Spring Cabbage is planted approximately 500,0000 acreage a year in our country. But the production of spring cabbage always suffered from climatic impact and directly result to vernalization, flower bud, just approaching to the premature bolting. In this paper, Using high generation of inbred lines LN-901(late bolting) and LN-905(early bolting) as the parents, We acquired the test materials, The cabbage bolting characters have been had genetic analysis and molecule marker which was linked to the bolting gene. The conclusions are as follows:1. All of the plant materials were cultivated in essay field. Sorting the bolting data of various groups and analysis knew that all of F1 group was bolting, the segregation ratio of bolting and unbolting plant was 1:1 in the F1×LN-901 backcross group; but in the F2 colonia, the segregation ratio was 254:70, and accordant with 3:1 by chi square test.The summary showed that the bolting trait correspond the Mendelian genetic law and early bolting was the dominant character to late bolting.2. Through major genes plus polygenes mixed inheritance model analysis showed that the cabbage bolting time was a quantitative trait controlled by several genes of the E model. That bolting time was controlled by two pairs of additive-dominance-epistasy major genes and aditive-dominance-epistasy polygenes. The heritability of major gene was 86.13% to 95.83%, the polygenetic was less than 3.46%, the influence of environment alteration was 4.15% to 8.05%, It showed that major genes played a decisive role in cabbage bolting time.3. By major genes plus polygenes mixed inheritance model analysis indicated that the cabbage flowering trait was controlled by several genes of the D model. That flowering time was controlled by 1 plus additive-dominance-control of epistatic genetic model. The heritability of major gene was 83.10% to 90.54%, the polygenetic was less than 6.13%, the impact of environment alteration was 1.04% to 13.32%. The result showed that major genes played a decisive role in inheritance of cabbage flowering time.4. In this experiment,90 pairs of SSR and 64 pairs of SRAP primers were used to amplify the DNA of parents and F1 groups. The results of amplification and seletcing showed that 27 pairs of SSR had amplified stable and repeatability fragments between parents and F1 group, which was 30% of the total primers, amplified a total of 264 stable bands, each pair of primer could amplify 9.8. There were 11 pairs which could amplify 112 differential bands. The percentage of polymorphism was 42.4%. The average polymorphism of each pair was 10.2. There were 14 pairs of SRAP primers which could amplify stability of difference bands in the parents and F1 generation, which was 18.8% of the total primers, amplified 79 stable bands, each pair of primer was 5.6. There were 3 pairs could amplify 26 polymorphism stripes, the polymorphism ratio was 32.9%. The average polymorphism band was 1.9.5. Using the polymorphism primers to screen the last primers through mix gene pools, In the 27 paris of SSR primers, only one pair of primers (O111-E03) could amplify stable bands in the parents and mix gene pools, and found that the special band was linked to the late bolting gene, the size was about 360bp, through Mapmaker 3.0 software, we got the genetic distance that linked to resistance bolting genes was 4.4cM. There was only 1 pair of SRAP primers could amplify stability belts in mix gene pools and the unusual belt was found in easy bolting gene pool. |
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