Studies On Artificial Infection And Immunization Of Parvovirus From Monkey

Parvovirus is a kind of DNA virus which is very wide distribution in nature. There are nearly 20 kinds of viruses in the genus Parvovirus within the family Parvoviridae,includingCanine parvovirus (CPV),Feline panleukopenia virus (FPV),Mink enteritis virus (MEV),and the Aleutian disease virus (ADV),etc. Simian parvovirus(SPV) is isolated from cynomolgus monkeys with severe anemia.The clinical manifestations of infected monkeys was very similar with that humans infected by Humans parvovirus B19 (HPV B19). Our laboratory isolated a strain of Parvovirus from monkeys with severe and infectious diarrhea.The the clinical symptoms of infected monkeys was similar to that of dogs and cats by parvovirus.VP2 gene sequence analysis showed that the homology of nucleotide sequence of this parvovirus from monkey was 98.75% with that of FPV and 98.15% with that of CPV.Infection in monkeys showed that the host range and pathogenicity of parvovirus were constantly changing with the gene mutation. how did this parvovirus from monkey break inter-species barrier and whether can it be able to infect humans cross the species barrier are still unclear. There have not been researches for its pathogenicity, host range, antigenicity, immunogenicity and serological surveys,etc. at home and abroad. Therefore. In this study we obtained a strain of parvovirus from monkey by isolated and identified of virus.Then we used it to artificially infecte cats, dogs, cynomolgus monkeys, ferrets, mink, and Netherlands rabbits of 1-2 months old with HI antibody to parvovirus less than1:2. Each animal were divided into two groups, three for the experimental group and three for the control group.After the experiment,we can preliminary learn the pathogenicity and host range of this parvovirus from monkey; At the same time, in order to know the infections of parvovirus from monkey in China, antibody survey to this parvovirus was carried out in monkeys by HA-HI.The 160 sera samples collected from captive monkeys in Beijing China during 2010 to 2011;In addition,in order to know the antigenicity and immunogenicity of the parvovirus from monkey, in this study we also developed its inactivated vaccine and the immunization study of this vaccine have been done.The results of this study confirmed that this parvovirus from monkey is a velogenic strain and it can break the species barrier to infecte monkeys, dogs and cats; antibody survey showed that the HI antibody positive rate to Parvovirus form m onkey is 58.1 % in monkeys.We proposed that the captive monkeys had been infected by Parvovirus in Beijing China. The immunization experiment results confirmed that this parvovirus from monkey has ideal immunogenicity and antigenicity.Its inactivated vaccine could induce specific immune responses against parvovirus form monkey after immunization to monkeys. This study will be a foundation for further identifying the infected animals range of the parvovirus form monkey, and for carrying out its epidemiological investigation and immunizationlaid. Whether is the parvovirus from monkey a variant from FPV or CPV, and how canit span cross species barrie to infect monkeys and occurrence pathogenic mutations still need further studies.