Isolation And Identification Of Antigenic Variants Of Canine Parvovirus And Preparation Of Anti-canine Parvovirus Monoclonal Antibody

Canine parvovirus (CPV) is one of the smallest and simplest singal strand DNA virus, which can cause contagion in dogs and several wild carnivorous species. CPV belongs to par voviridea and there are mainly two kinds of structural capsid proteins, VP1 and VP2. The complete sequence of VP2 gene which can determine the speciality of the antigen and host is 1775 bp long.In this study, CPV was isolated from faecal sample of dogs showing clinical symptom, and common virus identification and molecular biology identification were carried out. An antigenic CPV monoclonal antibody strain had been screened to study the mutation of CPV and laid the foundation for the identification and treatment of this disease.1. VP2 gene cloning and its sequence analysisWe used primers designed according to the Genebank sequences to clone VP2 gene from the canine faecal genome. The sequence of the DNA fragment was aligned and analyzed for the identification of the antigenic and evolution. The result showed that there were 5 of CPV-2b,1 of FPV and 10 of CPV-2a in 16 seqenced samples and homogeneities were above 98%.2.isolation and identification of canine parvovirus5 strains of canine parvovirus were isolated and identified by common virus identification and molecular biology identification, which could be divided into CPV2a and CPV2b type.3.anti-CPV monoclonal antibody preparationWe used CPV as antigen to screen out a hybridoma srain named C-1-A9 which could secrete antibody steadly by hybridoma technique and attempted to apply colloidal gold technique to prepare test paper for the clinical use.