Extraction And Purification Of Polyphenols From Carga Cathayensis Sarg Peels

In order to make full use of carya cathayensis sarg, polyphenols was extracted fromcarya cathayensis sarg peels and the optimum extracting method and conditions werecomfirmed though single factor and response surface methods. Microfiltration membraneand ultrafiltration membrane were employed to separate and purify leach liquor of cargacathayensis sarg peels. The main results were shown as follows:1. Response surface methodology was employed to optimize the extraction condition ofpolyphenols from carga cathayensis sarg peels treated with cellulase. On the basis ofone-factor-at-a-time experiments, a four-factor, three-level quadratic general rotaryunitization design was employed to establish a quadratic polynomial mathematical modelthat describes extracting rate as a function of four process conditions, and the establishedmodel was analyzed by drawing response surface and contour plots. The optimal extractionconditions were determined as follows:4U/g substrate at pH6and70℃for70min. Underthese conditions, the extracting rate of carga cathayensis sarg peels was predicted to63.43%and observed to61.72%with a relative error of2.77%.2. Leach liquor of carga cathayensis sarg peels were filtrated by inorganic ceramicmembrane and polysulfone hollow fiber ultrafiltration membranes, the effectivecomponent was enriched and the un-wanted larg molecules were removed in this method.The optimum operation were also determined in these experiments, such as the inorganicceramic membrane pore diameter, the average intercept molecular weight of polysulfonehollow fiber ultrafiltration membranes. The result showed that the purity of polyphenolsfrom carga cathayensis sarg peels can reach81.6%3. Compared with HPLC chromatogram of the product of polyphenol of carga cathayensissarg peels and HPLC chromatogram of leach liquor of carga cathayensis sarg peels,analyse the effect of spray drying method. The result showed that spray drying is aeffective method for polyphenols from carga cathayensis sarg peels. A HPLCdetermination of polyphenol was established. Polyphenols were separated very well onAngilent TC-C18colum by use of Acetic acid:water(2:98) and Acetonitrile as mobilephase, inject5μl under the detection wavelength of280nm and the flow rate of0.8ml/min.