The Effect Of Sea Cucumber And Its Bioactive Components On Hyperuricemia

The sea cucumber is an important food and drug resource which contains avariety of bioactive components. The sea cucumber is proved to exhibit variousbiological activities, including anti-tumor, antifungal, antioxidant,immunomodulatory, lowering lipid. In this paper, the sea cucumber and its bioactivecomponents are used to investigate the effect on uric acid metabolism infood-induced hyperuricemia mice and spontaneous obesity (db/db) mice. And themechanism is preliminary studied. The main researches in this thesis are listedbelow:1. The hyperuricemia model is induced by dietary yeast extract powder, and thestudy of sea cucumber saponin (SSC) and no-saponin residue found that both of thempossess a potent anti-hyperuricemic effect on yeast extract powder inducedhyperuricemia. After14days’ feeding, the SSC low dosage group (0.05%SSC) andSSC high dosage group (0.1%SSC) decreased serum uric acid level by53.1%(P <0.01) and55.6%(P <0.01) respectively. The no-saponin residue also could loweringuric acid (P <0.01) in day9, however, it doesn’t show significant difference in day14.The further study shows that the anti-hyperuricemia effect of saponin and no-saponinresidue is related to the inhibition of the hepatic xanthine oxidase (XOD) andadenosine deaminase (ADA) activities. The study also shows that ecxept SSC, thereare other bioactive components in sea cucumber could inhibit hyperuricemia2. Then, the effect of sea cucumber saponin, polysaccharides as well asPearsonothuria graeffei (Pg), Apostichopus japonicus (Aj), Cucumaria frondosa (Cf)and Isostichopus badionotus (Ib) on uric acid metabolism in yeast extract powderinduced hyperuricemia mice were investigated. And the mechanism is explain in genetranscription levels. The experiment shows that all of them could significant loweringserum uric acid after14days’ feeding, the serum uric acid level are decreased by14.9%(P <0.05),20.7%(P <0.01),16.1%(P <0.05),20.1%(P <0.01),12.2%(P < 0.05) and25.3%(P <0.01) respectively. And all of them could inhibit hepatic XODand ADA activities. Furthermore, the sea cucumber saponin and polysaccharides arefound to down-regulate the mRNA levels of hepatic XOD and ADA, renal glucosetransporter9(GLUT9) which is the important transporter in the process of thereabsorption of uric acid. Moreover, the sea cucumber saponin and polysaccharidesup-regulate mRNA levels of renal organic cation and carnitine transporters (OCTs andOCTNs) might involved in renal protective action.3. Finally, the obesity (db/db) mice were treated sea cucumber saponin—EA for6weeks. Dietary EA could significantly decreased serum uric acid level by18.1%and12.7%respectively. Furthermore, EA is found to up-regulate the mRNA levels ofrenal organic anion transporter1(OAT1), increase the secretion of uric acid. And theup-regulate mRNA levels of OCTs and OCTNs shows the improving of renal function.The HE stains and electron microscopy of renal cortex in db/db also prove thatEAcould recoverable renal structure and protect renal. However, the activities andmRNA levels of hepatic XOD and ADA are elevated by EA.In this study, we investigated the effect of the sea cucumber and its bioactivecomponents on hyperuricemia mice, and made a preliminary study of the mechanism.These provide a scientific basis for the comprehensive utilization of sea cucumber.