Synthesis And Property Research Of Carboxylated Magnetic Polymer Microspheres

Magnetic polymer microspheres are the combination of inorganic magnetic materialand organic polymer (size distribute in microns level or below). Functionalized the surfaceof the magnetic microspheres with rich content of the different functional groups throughthe surface modification. Thus they have higher coupling efficiency with avidin/antibodyand other biomoleculars. Since Functionalized magnetic microspheres have a lot ofadvantages such as powerful magnets, super paramagnetic and lots of functional groups, ithas a very wide range of applications in targeted drugs and immune determination, etc.In this study, microspheres were prepared by microemulsion polymerization. Thenfunctionalized the Surface of microspheres with-NH2groups by chemical modificationmethod, and in the meantime covering the microspheres with silica. Finally added theactivator to promote the reaction between the microspheres modified with amide groupsand poly(ethylene glycol) bis (carboxymethyl) ether. The carboxylated microspheres wereprepared successfully. In the experiment process, Optical microscopy and ScanningElectron Microscopy(SEM) is used to examine the morphology, single dispersion andparticle size distribution. Energy Dispersive X-Ray Spectroscopy (EDX) is used to test thecontaining elements. Thermal Gravity Analysis (TGA) and Vibrating SampleMagnetometer (VSM) is used to measure the magnetic contents, hot stability and themagnetic properties. Fourier Transform Infrared Spectrometer (FTIR) specifies thecovalent bond of the microspheres. Conductometric titration is used to analyze the amountof–NH2and–COOH groups on the microspheres. Fluorescence microscope is used toobserve the conjugate efficiency of the microspheres with avidin/antibody.