Study And Application Of Fluorone Reagent-Mo(VI) As Spectral Probe For Protein Determination In Microemulsion

The quantitative analysis of proteins is very important in bioanalysis and clinical analysis. It is an active field in bioanalysis to develop new methods of high sensitivity. In this paper, the progress of analytical technique and quantitative determination of protein has been reported in detail, as well as the binding mechanism of protein and micromolecule by fluorescent spectrometry. On the basis of the complex as a spectral probe of protein determination, fluorone reagents and Mo(VI) complexes are introduced and formed a series of spectral and resonance rayleigh scattering methods. Those methods have been successfully applied to the protein determination in samples. At the same time, their mechanisms are also discussed by fluorescence method, including binding constant, energy transfer distance and the main sorts of binding force. Some novel achievements are obtained.This paper features in systematic, scientific and creative methods.1. Systematic featureIn this paper, the reaction conditions (such as acidity, medium, amount of developer et al) of tri-hydroxyl fluorone substituted with different groups and protein by resonance rayleigh scattering and spectrophotometric methods have been researched systematically, as well as the effects of substituents on the sensitivity of system. What′s more, the distances and energy transfer efficiencies between complex and protein in the media of micelle, microemulsion and water have been determined. And the results have given direct evidences to verify the solubilization of microemulsion.2. Scientific featureThe method mentioned in the paper has been successfully applied to the determination of protein in blood and human urine samples. And the binding distance and the energy transfer efficiency between tri-hydroxyl fluorone-Mo(VI) complex and protein are obtained based on the theory of Forster spectroscopy energy transfer. According to the thermodynamic parameters, the main sorts of binding force can be judged.