Proteomics Analysis Of Suaeda Corniculata Seedlings Under Salt-alkali Stress

The world with large area distribution by saline-alkali wasteland, and the land salinization has become one of the reasons why limit agricultural production. Suaeda is a typical euhalophyte, and Suaeda corniculata, an annual herb halophyte which is widely distributed in the saline-alkali soil of the Songnen plain in northeast China, has been ignored by people for a long time. To investigate the influence of saline-alkali Stress on S.corniculata seedlings, we use a combined physiological and proteomic approach, and the way of S.corniculata salt-tolerance is also discussed. In hopes of providing fundamental help to research the salt-alkali-tolerance mechanism of S.corniculata.After the salt(NaCl) or alkali(NaHCO3) stress treatment, we analyzed the changes of biomass, photosynthesis, chlorophyll fluorescence, and K+and Na+contents in the seedlings. Using comparative proteomic analysis of S.cornicidata under salt or alkali stress, revealed23differentially expressed proteins. These proteins are mainly involved in stress and defense, photosynthesis, ion transport, energy and metabolism, and protein metabolism.After analysis the experimental datas, we found that the low contents of Na+and Cl-have little effect on halophytes, but the high pH environment produced by HCO3-is more harmful, alkali stress may Influence photosynthetic efficiency, damage the stomata, destroy the mesophyll cells, and limit transpiration, then, it can reduce the photosynthetic electronic transmission, and make the PSII damaged, finally, it will inhibit the normal ion transport.In conclusion, S.corniculata may use the changes of stress and defense, photosynthesis, energy and metabolism, and ion transport, all taken together to influence the salt-alkali-tolerance of S. corniculata. This study may provide base information for researching the salt-tolerance mechanism of halophytes.