Archaeal Diversity And Community Structure In Marine Sediments From The Source Area Of Kuroshio

To study the archaeal diversity of deep-sea sediments from the Source Area of Kuroshio inWestern Pacific, we investigated the archaeal community structure in the sediments, which are all collected from the deep-sea area to the east of Luzon Island and Benham Plateau. Subsamples along the MUD1, MUD2and MUD3sediment core collected during the Western Pacific sharing cruise and the MD06-3047sediment core collected during the IMAGES XIV cruise with16S rDNA analysis.A total of208archaeal16S rDNA clones were examined and a total of40OTUs were obtained in the subsamples form the deep-sea area to the east of Luzon Island Phylogenetic results showed that the archaeal diversity in the collected samples was very diverse, and the obtained OTUs were grouped into Crenarchaeota and Euryarchaeota. The major group was Crenarchaeota (89.9%, of total archaeal clones), which was dominated by MGI (84.6%, of total archaeal clones). And Euryarchaeota contained6archaeal phylotypes, which all took up tiny percentage (0.5%-3.4%, of total archaeal clones).A total of260archaeal16S rDNA clones were examined and a total of56OTUs were obtained in the subsamples form the Benham Plateau. Phylogenetic results showed that the archaeal diversity in the collected samples was very diverse, and the obtained OTUs were grouped into Crenarchaeota and Euryarchaeota. The major group was Crenarchaeota (91.5%, of total archaeal clones), which was dominated by MGI (40.8%, of total archaeal clones). And Euryarchaeota contained5archaeal phylotypes, which all took up tiny percentage (0.4%-5.4%, of total archaeal clones).By comparing the different samples, we can conclude that archaeal diversity of deep-sea sediments from the north Source Area of Kuroshio is higher then the south ones. Lots of the north samples have the same resource sequence with the ones in tropical ocean, and lots of the south samples have the same resource sequence with the ones in cold water. These maybe due to the different ocean currents. That is to say the northern current is Kuroshio and the southern one is nearshore current. These data have important implications for our understanding of archaeal community in deep-sea sediments from the Source Area of Kuroshio in Western Pacific and basis for the future study on the relationship between archaea and environment.