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The Ultrastructure Study Of The The Embryo Sac Of Day Lily (Hemerocallis Curma Baroni) Before And Atfer Fertilization

Posted on:2013-03-22Degree:MasterType:Thesis
Country:ChinaCandidate:Y C ZhaoFull Text:PDF
GTID:2230330374453100Subject:Botany
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In this paper, The electron microscope technique was used in day lily (Hemerocalliscitrina Baroni) of the mature embryo sac and fertilized embryo sacs of theultrastructural features of research, as the day lily embryological studies provideinformation. The main results are as follows:1,Synergid degeneration process. Early embryo sac two synergid cells exist at thesame time, and in a form not distinguish. The pollen tube into a cell, this helps the cellbegins to degenerate, the nuclei are irregular, rely on the one side of the egg cell,nuclear chromatin condensation integrated highly helical winding of chromatin, theemergence of a large number of lipids, organelle degradation only contours, we candistinguish the endoplasmic reticulum and mitochondria. When the cell cytoplasm ishighly opaque, another useful cell begins to degenerate, the degradation process and adegradation of the help of cells of the same. In cells, the egg cell and the central cellbetween the opaque electron dense bodies. Filiform apparatus still exists, but the formhas changed little to near the micropylar end, and the inner electron transparent bodyincrease. Night latch help cells to zygotic period will be degraded completely.2,After the egg cells before fertilization. Egg cell metabolism is help cell is low.Egg cell the chalazal cell wall before fertilization with honeycomb, egg cell nucleuslocated in the chalazal end, prominent nucleoli, no obvious nuclear vacuole in thepresence of. Before fertilization oocytes without apparent large vacuoles, but with alarge number of small vacuole, organelle is a perinuclear distribution around the state,mitochondria and amyloplast more, in the vacuole side accompanied by a large numberof lipid droplets, other organelles of rare. Homozygous for the formation of cell wall isa gradual process, honeycomb wall to the complete wall. After fertilization, zygotedormancy period, nucleolus becomes small, weakening polarity, cell tends to round inshape, and the shape of small were metabolically inactive state.3,After the central cells before fertilization. Most of the central cells beforefertilization extremely nucleus has fusion secondary nucleation, secondary nucleationis close to the oocyte chalazal end. There are large and dense nucleoli micro zone andthe apparent particle area without obvious nuclear vacuole in the presence of. Cytoplasm around the nuclear distribution state, other main body, fat body,endoplasmic reticulum, mitochondria and Golgi apparatus. And around the nucleardistribution of small vacuoles. The secondary nucleus, forming the primary endospermnucleus after fertilization. The primary endosperm nucleus nuclear irregular state,cytoplasmic organelles increased in number, the decrease in the number of fat plastid,the increase in quantities, mitochondrial cristae, surface cells in metabolically activestate.
Keywords/Search Tags:Lily (Hemerocallis citrina Baroni), ultrastructure, synergid, egg, centralcell, zygote, the primary endosperm nucleus
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