Effect Of Limited Fluid Resuscitation On The Protection Of Lung In Rats With Uncontrolled Hemorrhagic Shock

Objective:To investigate the effects of limited fluid resuscitation on the Lung tissue morphologyin、inflammatory response and apoptosis in rats with uncontrolled hemorrhage at the early stage.Mainly according to finding out the differences of the level of pathological examination of HE-stain 、the expression of NF-кB and ICAM-1、the situation of cell apoptosis between different ways of fluid resuscitation. We can get a conclusion that the limited resuscitation can contribute to the protection of the lung, from which we can get a pathophysiological theory which can help building the theoretical foundation of the clinical practice.Methods: Sixty SD rats were equally randomized into 4 groups: blank control group(C),limited resuscitation group( LR), conventional fluid resuscitation group(TR) and non-resuscitation group( NR),each group contains 15 rats, after being resuscited, they would be killed after 2 hours.The ultrastructure in lung tissue was observed by electron microscope,the protein levels of NF-кB in lung tissue was determined by enzyme-linked immunosorbent test, immunochemistry staining was used to detect the expression of ICAM-1 on lung while the apoptosis of lung was tested by means of TUNEL.Results:When the resuscitation was completed,the level of pathological examination of HE-stain 、the expression of NF-кB and ICAM-1、the situation of cell apoptosis show that the limited resuscitation group(LR)and traditional resuscitation group(TR)were lower than none resuscitation group( NR) while apparently higher than the control group(C)(P<0.01);LR group was lower than TR group on the pathological examination of HE-stain and the reflexing of cell apoptosis(P<0.01),and is also lower on the expression of NF-кB and ICAM-1(P<0.05).Conclusion:The limited fluid resuscitation can help reducing the responding of NF-кB and ICAM-1,releasing the pathological damage of lung tissue and the level of cell apoptosis,which may play the role of protecting the lung from further damage.