| Objective: The prostate is an important accessory gland of the malereproductive system, located between the bladder and the urogenitaldiaphragm,surrounding the the beginning part of the urethra. It secretes amilky prostatic fluid, an important part of the semen, accounting for the1/10-1/3of semen volume.The role of prostatic fluid is to promote thefertilized eggs to generate, stimulate sperm motility, and promote semenliquefaction, improve the survival rate of sperm, so the prostate plays animportant role in male reproductive function. Diabetes is a chronicdisease,which is serious harm to human health hazards such as cardiovascular,kidney disease, retinopathy. A large number of human and animal experimentshave confirmed that diabetes can lead to the testis and epididymis weightdecreased, testicular structural disorder and reduced semen quality, as well asmale erectile dysfunction.So far, The research of the effect of diabetes onprostate is less.This experiment study the impact of diabetes on rat prostatetissue and IGF-1expression on prostate.The purpose is to explore the possiblemechanisms of the impact of diabetes on prostate from the perspective of themolecular level, the theoretical basis for new therapeutic approach for diabeticmale reproductive disorders.Methods:35male Sprague-Dawley rats (200-250g) were randomlydivided into two groups after one week feeding: normal controlgroup(NC,N=15), diabetes model group(DM,N=20).Diabetic rats wereindused by using the STZ single abdominal cavity injection and the dosagewas65mg/kg,isochoric citric acid buffer solution for control group.Twogroups were given the ordinary animal feeds that were bought fron HebeiMedical College Experimental Aninal Center. Fasting blood glucose(FBG)wasmeasured after injecting the STZ for72hours. Diabetic model was successfully built when BG>16.7mmol/L. Two groups of animal tail venousblood glucose were measured when9weeks after diabetic model issuccessfully built,there are not the rats whose blood glucose was lower than16.7mmol/L.Experiment was carried out when9weeks after diabetic model was builtand before the experiment metabolism cage24hours amounts of urine wasmeasured.10%chloral hydrate anaesthesia,whole prostates was cut off.Prostates wet weight was measured and all specimens were fixed immediately.Using Haematoxylin-Iraq red(HE) dyes,morphological change of diabetes ratsProstates tissue was observed under microscope. IGF-1expression inprostates were measured by immunohistochemical staining.Under microscopethe results were observed,it was positive result in the prostates when yellowishbrown pellet emerges.The expression of IGF-1at protein level was detectedby IHS (immunohistochemistry)Statistics processing:Uses the SPSS13.0softwares to carried onindependent-samples T-test,it was thought significant difference when P<0.05.The experimental data was presented as mean±standard deviation.Results:1Blood glucose, insulin,body weight, metabolism cage24hoursamounts of urine and prostates wet weight and prostate index of rats in eachgroup:72hours after abdominal cavity injecting STZ,BG of rat has alreadyelebated.When9weeks, BG of diabetic group(n=20) was obviously higherThan that of the control group(n=15)(29.02±2.71vs4.73±0.62)(mmol/L).(P=0.00vs contral)Insulin of diabetic group(n=20) was obviously lower than that of controlgroup(n=15)(7.03±0.68vs23.24±1.93)(uIU/ml)(P=0.000vs contral).Body weigt droped obviously. Body weight of diabetic group(n=20) wasobviously lower than that of control group(n=15).(249.40±11.89vs377.07±15.39)(g),(P=0.000,vs contral).When9weeks,the metabolisn cage24hours amounts of urine of diabetic group(n=20) was obviously higher than that of control group(n=15)(88.30±5.23vs20.80±2.00)(ml),(P=0.000,vs contral)Prostate wet weight of diabetic group(n=20) was obviously lower thanthat of control group(n=15)(201.29±24.81vs934.15±165.35)(mg),(P=0.000,vscontral).Prostate index of diabetic group(n=20) was obviously lower than that ofcontral group(n=15)(0.80±0.06vs2.47±0.34),(p=0.000,vs contral).2HE and Immunohistochemical sdudiesLight microscopy showed normal rat prostatic epithelial for singlecolumnar, fold more, whereas diabetic rats prostatic epithelial cell atrophy,reducing the height, flat, fold reduction becomes more empty, catheter,catheter reduced secretion。Normal prostate epithelial cells layer visible on IGF protein expression,brown, glandular epithelial expression of IGF-1in group DM was weaklypositive, showed a punctate distribution, statistical analysis revealed twodifferent.(5.33±1.84vs3.00±1.92)(P=0.001)Conclusion:1,IGF-1in normal prostate epithelium showed positive expression.2,The expression of IGF-1in diabetic prostate epithelial huve reduced.3,Diabetes mellitus causes prostate atrophy, which may affect the functionon male reproductive effects4,The reduction of expression of IGF-1in prostate epithelial in diabetesmay be one of the factors at prostate lesions. |
PDF Full Text Request