Dan Hon Injection Of Obstructive Jaundice Rats IL-2and SIL-2R Express Influential Role

Objective:This experiment will build OJ animal model of rats, application Dan hon injection intervene, dis-cusses Dan red injection to obstructive jaundice rats lymphocytes IL-2and sIL-2R influential role. Further clarify the OJ pathogenesis, clinical application for Dan hon injection treatment OJ to provide the theory basis; At the same time seeking peri operative improve the immune function of pa-tients with obstructive jaundice, or shorten the immune function recovery time drugs have clinical practical value.MethodSD female rat weight at200-220g between animals by Dali college room provided), will anim-als were divided into three groups, each group of only15. A group for JiaShouShu group; Group B for bravery manager ligation group, the total work0th bravery thread double ligation, postoper-ative day3began, every day in the abdominal cavity injection saline1mL, for two weeks; Group C for bravery manager ligation plus treatment group, operation methods with group C, the third d-ay after, every day in the abdominal cavity injection Dan red injection1mL, for two weeks. The e-xperiment end, all animals are heart cavity, take the liver tissue samples from blood for testing.The results(1). Serum alanine amino shift enzyme (ALT), aspartic acid amino shift enzyme (AST), total bi-lirubin (TBIL) and bile acid test(TBA) results:Group A serum ALT, AST, TBIL and group compared with B TBA, group B in the serum ALT, AST, TBIL and significantly higher TBA (P<0.05), signific-ant difference was statistically significant,; A and C group compared, two groups of serum ALT, AST, TBIL and content are statistically significant differences TBA (P<0.05); Group B group com-pared with C, B group of serum ALT, AST, TBIL and comparison group C TBA was higher, the diffe-rence was statistically significant (P<0.05).(2) serum IL-2test results:group A serum IL-2group compared with B, B group of serum IL-2content significantly (P<0.05), significant difference was statistically significant,; Group A group compared with C, two groups of serum IL-2content are statistically significant difference (P<0.05); Group B group compared with C, B group of serum IL-2significantly lower than the group C, the difference was statistically significant (P<0.05).(3) serum sIL-2R test results:group A serum sIL-2R group compared with B, B group of serum IL-2content increased significantly (P<0.05), significant difference was statistically significant,; Group A group compared with C, two groups of serum sIL-2R content are statistically significant difference (P<0.05); Group B group compared with C, B group of serum sIL-2R was higher than the group C, the difference was statistically significant (P<0.05).(4) the liver tissue pathology change:group A normal liver tissue structure; Group B liver cell pat-ch of necrosis, a large number of inflammatory cells infiltration, when hepatic fibrosis, normal lob-ular were rebuilt false flocculus form. Group C liver cell mild edema, some inflammatory cells infi-ltration, a few collect abbacy lobules appear small bile duct kind of surrounding epithelial cells, an-d group B liver tissue is the morphological structure obviously improved.Conclusion:(1) Dan hon injection can reduce obstructive jaundice host of serum total bilirubin and bile acid, and protect the liver cells.(2) Dan red injection can improve the immune function of obstructive jaundice.