Research On The Influence Of Moringa Composite Enteral Nutrition On The AIDS Patients' Intestinal Microecology

Part ⅠResearch on the change of intestinal microecology in AIDSObjective To analysize the change of Acquired Immune Deficiency Syndrome (AIDS) patients' intestinal microecology which helps explore the influence of AIDS to intestinal microflora, and to explore its mechanism.Methods Collected the feces of patients with AIDS (n=30) and healthy volunteers (n=30). A set of16S rRNA gene group of species-specific primers for Bifidobacterium spp., Lactobacillus group, Escherichia coli, and ddl gene-targeted species-specific primers for Enterococcus faecalis and feces Enterococcus were designed. Bacterial genome DNA extracted from fecal samples were quantified by SYBR Green Ⅰ real-time fluorescence quantitative PCR to analyze bacterial amounts, analysis the difference of the two groups of gut microflora.Results The level of Bifidobacterium spp.(9.17±1.29vs.5.75±1.54) and Lactobacillus group (8.03±1.23vs.5.04±1.04) in AIDS group were significantly lower than those of healthy control group, whereas Escherichia coli (4.67±0.80vs.7.59±1.24), Enterococcus faecalis(4.56±0.87vs.6.09±1.08) and feces Enterococcus (5.10±0.92vs.9.48±0.73) were significantly higher than those of healthy control group, There was significant difference (P<0.05).Conclusion The intestinal microflora of AIDS patients have obviously changed.These data underline that the intestinal microflora of AIDS patients is confusion. Part IIThe change of AIDS patients'intestinal microecology before and after the treatment of Morigna composite enteral nutritionObjective To analysize the Acquired Immune Deficiency Syndrome(AIDS) patients'intestinal microflora before and after the treatment of Morigna composite enteral nutrition which helps explore the influence of Morigna composite enteral nutrition to AIDS patients'intestinal microflora. To provide a new theoretical basis for the clinical treatment of AIDS infection and other complications.Methods The AIDS patients were randomly divided into nutrition treatment group(include common enteral nutrition group, Morigna group and Morigna composite enteral nutrition group) and control group, separately collected the feces of patients after nutrition treatment for2weeks and4weeks. Bacterial genome DNA extracted from fecal samples were quantified by SYBR Green I real-time fluorescence quantitative PCR to analyze bacterial amounts.Results After the nutrition treatment for4weeks, the Bifidobacterium spp.,and Lactobacillus in nutrition treatment group were significantly higher than those of control group(P<0.05), the Bifidobacterium spp.,and Lactobacillus in Morigna composite enteral nutrition group were significantly higher than those of Morigna group and in common enteral nutrition group(P<0.05); the Escherichia coli, Enterococcus faecalis and feces Enterococcus in nutrition treatment group were significantly lower than those of control group (P<0.01), in Morigna composite enteral nutrition group were significantly lower than those of in common enteral nutrition group(P<0.05).Conclusion Morigna composite enteral nutrition have a significant affect on rise of AIDS patients'intestinal probiotics, antagonist intestinal opportunity pathogenic bacteria, significantly ameliorate the AIDS patients'intestinal microflora.