Expression And Clinical Significance Of PTEN And Nm23in Endometrial Carcinoma

OBJECTIVE: To evaluate the expression of PTEN gene and nm23gene innormal endometrial, endometrial atypical hyperplasia, and endometrial carcinomatissues. The relationship of clinicopathologic feature and correlation of the expressionof PTEN gene and nm23in endometrial carcinoma was study, and to explore the roleand mean of them in the generation, development, treatment and prognosis ofendometrial carcinoma.METHODS: The expressions of PTEN and nm23in62specimens ofendometrial carcinoma,33specimens of endometrial atypical hyperplasia and15specimens of normal endometrial were detected by S-P immunohistochemistry. Therelationship of the clinical pathology features and correlations between theexpressions of genes in endometrial carcinoma was studied. Dates in this study wereevaluated with X₂test, Fisherman exact test, Spearman correlation test usingSPSS17.0software.RESULTS:1.The positive expression rates of PTEN in normal endometrium,endometrial atypical hyperplasia and endometrial carcinoma tissues were reducedgradually (86.7%,69.7%, and33.9%,X₂=28.745, P<0.001). The positive rates ofPTEN in endometrial carcinoma of histological differentiation were G1:52.2%,G2:29.2%, G3:13.3%,(X₂=6.275, P=0.040), respectively. The expression of PTENwas correlated to histological differentiation and myometrial invasion (X₂=9.692,P=0.008), but not to pathologic stage (X₂=0.764, P=0.682) and lymph nodemetastasis (X₂=0.021, P=0.884).2. The positive expression rates of nm23in normal endometrium, endometrialatypical hyperplasia and endometrial carcinoma tissues were reduced gradually(93.3%,69.7%,54.8%, X₂=31.530, P<0.001). The positive rates of nm23inendometrial carcinoma of histological differentiation were G1:78.3%, G2:50.0%, andG3:20%, respectively. The expression of nm23was correlated to histologicaldifferentiation (X₂=12.450,P=0.002), operation-pathological stages (X₂=9.692,P= 0.008) and lymph node metastasis (X₂=13.082, P=0.001), but not to myometrialinvasion (X₂=0.214, P=0.898).3. The expression of PTEN was positively correlated to that of nm23inendometrial carcinoma (P<0.001, r=0.479).CONCLUSION:1. The expression of PTEN gene and nm23gene plays animportant role in carcinogenesis and development of endometrial carcinoma.2. The PTEN gene mutation may occur in early stage of endometrialcarcinaoma.3. Comprehensive analysis of PTEN gene and nm23gene is benefit to improveearly diagnosis, judge malignant degree prognosis of endometrial carcinoma andtherapy.