| Objective: Henoch-schonlein purpura nephritis is one of commonsecondary glomerular diseases in children period, and its incidence isincreasing year by year. Its incidence mechanism mainly involves immunesystem disorders, and at the same time have cell factors and inflammation inthe blood coagulation and fibrinolytic disorders and gene polymorphismchange also plays an important role. This study observes that having immuneadjustment function and liver kidney embellish lung function of Huaiqihuanggranules influence on children HSPN serum T cells subsets (CD3+, CD4+,CD8+and CD4/CD8+), urine Renal Injury Molecules-1(Kim-1) and urinesP-Selectin, and to learn whether Huaiqihuang granules could regulatechildren's immune function, cell factors and coagulation system..Methods:1the research object and the experimental group: Choose in November2010to October2011pediatric renal medicine in our hospital in accord with thecurrent domestic HSPN formulated diagnostic criteria (2000, zhuhai) ofchildren of54, the male35patients, female19cases, aged3to14years old,HSPN according to the clinical manifestations of divided into: group A issmall proteinuria and (or) hematuria,group B is a lot of proteinuria. Group Aand group B respectively in accordance with the principle of random pointsA1(16cases, male, female,10cases of6cases, mean age7.6years), B1(10cases, male in7cases,3cases of female, with an average age of8years old)for the treatment group, A2(18cases, male, female11cases in7cases, meanage7.4years), B2(10cases, male in7cases,3cases of female, mean age7.9years) as control group. For children are treated first, prior to admissionapplication hormone and other immune inhibitors, except two weeks prior toadmission once used kidney drug toxicity and all kinds of the causes of acute or chronic renal damage children. After admission of A and B both groups toconventional therapy (including prednisone, vitamin C, rutin, strong overflow,dean of chocolate, and nephritis, resistance to infection and recovery), basedon this, the treatment group add with Huaiqihuang granules, less than3yearsold,5g bid; More than3years old,10g bid; Treatment a month. Another setof health in15cases, the male in7cases,8cases of female, with an averageage of6.8years. The same period choose outpatient age, gender matched thehealthy check-up of children, no rheumatism, asthma history.2test index and experimental methods: The operation flow cytometrydetermination T cells subsets, with double antibody sandwich ABC-ELISAmeasuring element and urine urine P choice of kidney injury molecules-1expression. Detection all kinds of index before conventional medicine andafter a month of treatment. The data from the statistical analysis softwareSPSS13.0statistical treatment, and a mean±standard deviation said, clinicaldata between the statistical methods for t test and analysis of variance, P <0.05represents a significant difference, P>0.05for no significant difference.Results:1The comparison of T cells subsets determination values in group A, group Band health group.1.1The comparison of before treatment CD3+, CD4+, CD8+and CD4/CD8+in group A1and group A2, the difference was not statistically significant(P>0.05). Group A1and group A2before treatment compared with healthygroup, the differences of CD3+, CD4+are statistically significant (P<0.05). Inthe group A1and group A2, CD4+determination value before and aftertreatment, the difference was statistically significant (P<0.05); The two groupsafter treating comparison, improve CD4+aspects, the treatment group than incontrol group (P<0.05). Group A1after treatment compared with health group,the difference of CD3+, CD4+, CD8+and CD4/CD8+were not statisticallysignificant (P>0.05), and group A2after treatment compared with healthgroup, CD3+, CD4+still have statistically significant (P<0.05). Visiblely,treatment group improve serum CD3+, CD4+better than the control group. 1.2The comparison of before treatment CD3+, CD4+, CD8+and CD4/CD8+in group B1and group B2, the difference was not statistically significant(P>0.05). Group B1and group B2before treatment compared with healthygroup, the differences of CD3+, CD4+, CD8+and CD4/CD8+are statisticallysignificant (P<0.05). In the group B1and group B2, CD3+,CD8+determination value before and after treatment, the difference was statisticallysignificant (P<0.05); the comparison of group B1and group B2after treating,the treatment group better than in control group in improving CD3+,CD8+aspects(P <0.05).2The comparison of urine Renal Injury Molecules-1(Kim-1) determinationvalues in group A,and group B.2.1The determination values of urine Kim-1before treatment are notstatistically significant in group A1and group A2(P>0.05). These two groupsof children after treatment compared with before treatment, urine Kim-1numerical are reducing significantly, and difference is statisticallysignificant(P<0.05); the comparison of urine Kim-1numerical after treatment,group A1in reduncing urine Kim-1better than group A2.(P<0.05).2.2The determination values of urine Kim-1before treatment are notstatistically significant in group B1and group B2(P>0.05). These two groupsof children after treatment compared with before treatment, urine Kim-1numerical are reducing significantly, and difference is statisticallysignificant(P<0.05); the comparison of urine Kim-1numerical after treatment,group B1in reduncing urine Kim-1better than group B2.(P<0.05).3The comparison of urine sP-Selectin determination values in group A,andgroup B.3.1The determination values of urine sP-Selectin before treatment are notstatistically significant in group A1and group A2(P>0.05). These two groupsof children after treatment compared with before treatment, urine sP-Selectinnumerical are reducing significantly, and difference is statisticallysignificant(P<0.05); the comparison of urine sP-Selectin numerical aftertreatment, group A1and group A2are not statistically significant(P>0.05). 3.2The determination values of urine sP-Selectin before treatment are notstatistically significant in group B1and group B2(P>0.05). These two groupsof children after treatment compared with before treatment, urine sP-Selectinnumerical are reducing significantly, and difference is statisticallysignificant(P<0.05); the comparison of urine sP-Selectin numerical aftertreatment, group B1and group B2are not statistically significant(P>0.05).Conclusion: Henoch-schonlein purpura nephritis exist T cells reduced, andthe immune function disorder, blood coagulation and fibrinolytic disorders andkidney damage. Huaiqihuang granules not only can improve the T cellsimmune function disorder, also has to protect the function of kidney, but toimprove blood coagulation and fibrinolytic disorder effect is not obviously.Huaiqihuang granules have little side effects, and it can be used as a treatmentfor the HSPN computer-aided drug. |
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