The Significance Of Urine MCP-1 And MIF In Children Henoch-Schonlein Purpura And Its Relationship With Nephritis

Purpose: To evaluate the clinical significance ofurinary monocyte chemoattractant protein-1(MCP-1)and macrophage migration inhibitory factor(MIF)in Henoch-Schonlein purpura(HSP)childrenwith nephritis.Methods:According to whether combined with Henoch-Schonlein purpura nephritis(HSPN),153 HSP children were grouped into HSPN group consisted of 81 HSPN children and HSP group consisted of 72 HSP children.HSPN group were further divided into mild proteinuria group(group A,TUPr<25mg/kg),moderate proteinuria group(group B,25mg/kg?TUPr<50mg/kg)and severe proteinuria group(group C,TUPr?50mg/kg)according to total urine protein in 24 hours(TUPr).Meanwhile,45 children with indirect inguinal hernia hernioplasty were selected as normal control group.The levels of urinary MCP-1 and MIFwere measured by enzyme-linked immunosorbent assay(ELISA).At the same time,levels of serum creatinine,blood urea nitrogen(BUN),urinary ?1-micro globulin(?1-MG),micro-albumin(mAlb),immune-globulin G(IgG),transferrin(TRF)and 24-h proteinuria(TUPr)assessments were performed to determine their associations with MCP-1 and MIF.Results: 1.55/81 of patients had abdominal pain(69.0%)were diagnosed with HSPN in comparison to 23/72(33.3%)of patients with HSP,this difference was statistically significant(p<0.05).2.The urinary MCP-1 and MIF concentrations in HSPN were288.02±150.71 pg/mL and 3.65±1.79 ng/mL respectively.The urinary MCP-1 and MIF concentrations in HSP were 80.14±46.13 pg/mL and 1.27±0.69 ng/mLrespectively.The urinary MCP-1 and MIF concentrations in controls were54.84±21.47pg/mL and 1.08±0.47ng/mLrespectively.The levels of urinary MCP-1 and MIF were significantly higher in HSPN group in comparison with HSP group and control group(p<0.01),but no significant difference was found between HSP group and the control group(p>0.05).3.The urinary MCP-1 and MIF concentrations in group Awere142.39±80.64 pg/mL and 2.04±0.81 ng/mL respectively.The urinary MCP-1 and MIF concentrations in group Bwere294.61±73.83 pg/mL and 3.47±0.77 ng/mLrespectively.The urinary MCP-1 and MIF concentrations in group Cwere472.14±90.98 pg/mL and 6.08±1.06 ng/mLrespectively.With the increase of TUPr,urine MCP-1 and MIFconcentrations in HSPN subgroups were obviously increasing trend,and the differenceswere statistically significant.4.The levels of urinary MCP-1 and MIF correlated positively with urinary?1-MG,mAlb,IgG,TRF and TUPr in HSPN,whereas negatively with the serum BUN and creatinine.Conclusion: Increased MCP-1 and MIF were found in children with HSPN correlated with proteinuria.MCP-1 and MIF may playan important role in pathological process of HSP renal lesion.Urinary MCP-1 andMIF could be used as suitable non-invasive biomarkers to provide valuable information not only for the diagnosis of HSPN,but also for evaluation of severity of renal damage.