| Objective: To observe the adiponectin and leptin level ofinsulin-resistant rats which was induced by high fat diet, test theexpression of adiponectin receptor and leptin receptor in the liver,andstudy the curative effect and the mechanism of Didangtang on theprevention and treatment of insulin-resistance. Insulin combinating toinsulin receptor play an important role in regulating GLU. If the numberof insulin receptors reduces or theri effect goes down, insulin could notplay its proper role in reducing insulin resistance. The fat cells are notonly the organs to reserve energy, but also to secret many cytokines.Cytokines play an important role in energy balance and metabo-lism.Most cytokines are risky factors leading to cardiovascular diseaseand IR.Some cytokines can prevent the function of IR, forexample:adiponectin,and some others lead to the occurrence of IR,suchas, leptin, TNF-α and so on.Didangtang produced from a work Shanghanzabinglun written byZhang Zhongjing,it is composed of rhubarb, peach kernel, Tabanus andleeches.It is an important prescription to treat the pattern of stagnatedblood with the function promoting blood circulation and eliminatingblood stasis. In this experiment, we used the method of high-fat food tocopy insulin resistance model. Didangtang was given to treat it. Thechanges of the above indexes in IR rats were observed to explore themechanism of Didangtang in inducing insulin resistance, and to providetheoretical basis for clinical application of Didangtang. Method:1Experimental animals:35health and clean level Wistar male ratswith weight about180g-220g were divided into5groups randomly, thecontrol group(C), model group(M), low-dose Didangtang group(L), high-dose didangtang group(H), Xue zhi kang group(X).2The administration of drugs: the rats of C group were fed withnormal diet, M group were fed with high cholesterol diet,and the rats ofC and M group were fed with distilled water (10ml/kg.d); L group werefed with high cholesterol diet and1g of Didangtang(crude drug/kg.d);Hgroup was fed with high cholesterol diet and2g of Didangtang (crudedrug/kg.d);X group were fed with high cholesterol diet and0.2g/Kg.d ofXuezhikang.3Indicators: All animals in five groups were fed as above methodsfor8weeks.At the end of the eighth week,blood was taken to measurebiochemical markers and serum adiponectin and leptin.The liver tissuewas taken promptly to measure AR2mRNA,OB-R mRNA.Results:1Effect of Didangtang on GLU,FINS,HOMA-IR,ISICompared with control group, FINS, HOMA-IR were increased,while ISI was decreased with statistical significance(P<0.01), itindicated that the IR models were copied succesfully; Compared withmodel group, Glu, FINS, HOMA-IR were decreased (P<0.05or P<0.01),while ISI was increased in low-dose group and high-dose group(p<0.01);Compared high-dose group with low-dose group, Glu, FINS, HOMA-IRwere decreased in high-dose group, while ISI was increased more thanthat in low-dose group, but there was no statistical significance(P>0.05);compared low-dose group and high-dose group with Xue Zhi Kanggroup, in high-dose group, Glu was decreased (p<0.05), ISI wasincreased (p<0.05), FINS,HOMA-IR were decreased more than Xue ZhiKang group, but there was no statistical significance(P>0.05).2Effect of Didangtang on TC, TG, LDL-C, HDL-CCompared with control group, TC, TG, LDL-C were increased (p<0.01), and HDL-C was decreased obviously in model group (p<0.01);Compared with model group, TC, TG,LDL-C were decreased(p<0.01),while HDL-C was increased obviously in low-dose group(p<0.01);Compared with model group, TG, TC, LDL-C were decreasedobviously (p<0.01), while HDL-C was increased in high-dose group(p<0.01);Compared high-dose group with low-dose group, in high-dosegroup TC,LDL-C were decreased more than that in low-dose group, butthere was no statistical significance(P>0.05),while high-dose groupHDL-C was increased more than in low-dose group, but there was nostatistical significance(P>0.05); Compared Low-dose and high-dosegroup with Xue Zhi Kang group, TC was decreased(P<0.05),TG,LDL-Cwere increased more than Xue Zhi Kang group, but there was nostatistical significance(P>0.05).3Effect of Didangtang on APN,LPCompared with control group, APN was decreased obviously(p<0.01),while LP was increased in model group(p<0.05); comparedwith model group, LP was decreased obviously (P<0.05) in low-dosegroup and high-dose group, APN was increased (p<0.01) in high dosegroup and low-dose group; Compared high-dose group with low-dosegroup, APN was more in high group than that in low-dose group, whileLP was decreased more in low-dose group than that in high-dosegroup,but there was no statistical significance(P>0.05); comparedlow-dose group and high-dose group with Xue Zhi Kang group, Inhigh-dose group APN was increased(p<0.05),while LP was decreasedmore than Xuezhikang group, but there was no statistical significance(P>0.05).4Effect of Didangtang on the expression of AR2mRNA,OB-R-mRNACompared with control group, The expression of AR2mRNAdecreased in model group(p<0.05)and the expression of OB-R mRNAwere decreased obviously in model group(p<0.01); Compared low-dose group and high-dose group with model group, the expression ofAR2mRNA was increased significantly in low-dose group and inhigh-dose group(p<0.01), the expression of AR2mRNA was increased(p<0.05),while the expression of OB-R mRNA was increased obviouslyin high-dose group, the expression of OB-R mRNA was increased morein low-dose group than that in model group, but there was no statisticalsignificance(P>0.05); Compared high-dose group with low-dose group,the expression of AR2mRNA, OB-R mRNA were increased more thanlow-dose group, but there was no statistical significance(P>0.05);Compared low-dose group and high-dose group with Xuezhi kang group,in high-dose group the expression of AR2mRNA, OB-R mRNA wereincreased more than that in Xuezhikang group, but there was nostatistical significance(P>0.05).Conclusions:1High cholesterol diet can copy rat model of IR, indicating thefunction of IR closely is related to diet abnormalities.2Didangtang can increase insulin sensitivity in IR rats.3Didangtang can decrease serum leptin levels and raise IR ratsserum adiponectin levels.4Didangtang can increase the expression of adiponectin receptorand leptin receptor in the liver tissue of IR rats. |
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