Human Blood Leukocytes KLF-4Expression Of The Downward Effect Of DVT Clinical Research

Objective:Access to PubMand and Gene Card database, locked deep in the period of the formation of DVT in rat femoral vein animal model of Affymetrix,230A cDNA gene chip Rat statistical research, the pre-screening for induction of deep vein thrombosis on the basis of significantly differentially expressed genesvenous thrombosis in the down-regulated genes KLF-4. Detection of KLF-4in normal human blood samples, blood clots do not form expression changes in the formation of blood samples of patients with blood samples of patients and deep vein thrombosis. Explore the relationship between the expression of KLF-4and deep vein thrombosis; to determine the transcription levels of human blood cells KLF-4as early diagnosis of deep vein thrombosis candidate molecular markers.Methods:1. The prevention of venous thromboembolism in the NICE guidelines (2012) and American College of Chest Physicians Association antithrombotic thrombo-prophylaxis clinical practice guide-the diagnosis of deep vein thrombosis (2012, section9) develop DVT in this studydiagnostic criteria:DVT diagnosis=risk assessment specialist risk factors)(the basis of risk factors+main clinical manifestations of+deep venous color Doppler ultrasound examination (Appendix2). March2010to March2012were included in the58cases, normal control group (n=20healthy volunteers), blood clots do not form a group (20cases of lower extremity long bone fractures or spinal fracture7d experts surgery without the formation ofpatients with thrombosis), thrombosis (18cases, the formation of DVT patients), Blood clots do not form a group and thrombosis for dealing with the group according Three groups were collected fasting venous blood samples, blood clots do not form a group collected on the first day after surgery, thrombosis group collected on the first day after diagnosis. Collection of blood samples, application primer5.0cited material design software people KLF-4design anti-transcription primers, will total RNA of each group blood cells anti-transcription is of cDNA, to GAPDH as within the reference to the use of semi-quantitative PCR KLF-4in clinical blood samples leukocytes in Situation.2. Processing,analysizing the experimental data,analyzed KLF-4expression and function of deep vein thrombosis,to determine the KLF-4transcription levelsof human blood cells can be used as a candidate molecular markers of early diagnosis and prognosis of DVT.Result: Can be seen from the semi-quantitative PCR plastic map and target gene and reference gene gray value ratio,the internal reference control GAPDH uniform size and uniform brightness,group C bands significantly higher than in group A and group B with a decrease in brightness,The gray values:A group(0.9803±0.0040),B group(0.9764±0.0039),C group (0.3751±0.0159),C group compared with A group and B group, P<0.05,the gap was statistically significant, B group compared with A group,P>0.05,the gap was no statistical significance.Conclusion:1. There are close correlations between KLF-4and DVT which maybe one of the main factors affect the biological state of deep vein thrombosis.2. KLF-4expression in the person of deep vein thrombosis in the blood down,so KLF-4detected in the blood can be used as molecular markers to predict the diagnosis of deep vein thrombosis.