Complement Receptor 1 Polymorphisms And Risk Of Late-onset Alzheimer's Disease

Objective:To define whether there is a relationship between Complement receptor 1 (CR1) gene polymorphisms and late-onset Alzheimer's disease (LOAD) in a Chinese Han population.Methods:254 LOAD patients and 357 healthy subjects matched for sex and age were recruited for the study. Two single nucleotide polymorphisms (rs6656401 and rs3818361) of CR1 and ApoE gene were detected using Matrix-Assisted Laser Desorption Ioniziation-Mass Spectrometry (MALDI-MS) and multiplex amplification mutation system PCR (Multi-ARMS).Result:The results revealed that there were significant differences in genotype (P=0.02) and allele (P=0.01) frequencies of the SNP rs6656401 but no in rs3818361 between AD patients and controls. The A allele of rs6656401 was associated with an increased risk of LOAD (P=0.01, OR=2.88). In the subgroup of APOE s4 non-carriers, both the A of rs6656401 and T allele of rs3818361 were observed to be significantly higher in case than in controls (P=0.002 and P=0.035, respectively). For rs6656401, the logistic regression analysis revealed that the (AA+AG) genotypes has a 2.4-fold increased risk compared with the GG genotype (P=0.049). Haplotype analysis identified the AT haplotype to increase the risk of LOAD (P=0.03, OR=2.44).Conclusions:The polymorphisms of CR1 genes are associated with LOAD in Chinese Han population. The A allele of rs6656401 and the AT haplotype may be independent risk factors for the LOAD.