Association Of Complement Receptor 1 Polymorphisms With Hepatitis B-Related Liver Disease

Objectives To examine the complement receptor 1 (CR1) gene polymorphism of rs3811381, rs2274567 in Guangxi population. To explore association between rs3811381, rs2274567 and hepatitis B (CHB), liver cirrhosis (LC), hepatocellular carcinoma (HCC) in Guangxi, which would provide new genetic markers and biological targets for the prevention and treatment of CHB, LC, HCC in Guangxi population.Methods Chain reaction-sequence specific primers (PCR-SSP) and chain reaction-restriction fragment length polymorphism (PCR-RFLP) were conducted to examine polymorphysms of CR1 in 100 patiens with CHB,71 patients with LC,228 patients with HCC and 227 healthy controls. The chi-square was used to analysis allele and genotype frequency distribution in the control group and case group differences. Odds ration (OR) and 95% confidence interval (95%CI) among different gentype and allele frequencies were calculated by logistic regression analysis, which were adjusted by age, gender, drinking, smoking and race. SHEsis software was conducted to construct the haplotype.Results1. Control group of CR1 rs3811381 and rs2274567 was out of HWE (P<0.05), the other groups were in HWE (P>0.05).2. There were CC、CG and GG genotypes in CR1 gene rs3811381. After logistic regression analysis by adjusted with gender, age, drinking, smoking and race, we found no association between rs3811381 polymorphism and CHB, LC, HCC susceptibility (P>0.05).3. There were AA、AG and GG genotypes in CR1 gene rs2274567. After logistic regression analysis by adjusted with gender, age, drinking, smoking and race, we found no association between rs2274567 polymorphism and CHB, LC, HCC susceptibility (P>0.05).4. When stratified by gender, we found the rs3811381 polymorphism of G allele may be risk factors for LC in Guangxi female, and the rs2274567 polymorphism of GG genotype may be risk factor for HCC in Guangxi male.5. When stratified by age, the CR1 rs3811381 site GG genotype increase the risk of HCC in people over 50 (p=0.017, OR=2.912, 95%CI=1.214-6.983), the CR1 rs3811381 site CG genotype and G allele decrease the risk of HCC in people under the age of 50 (p=0.037, OR=0.436,95%CI=0.199-0.952; p=0.019, OR=0.612,95%CI= 0.406-0.923); The CR1 rs2274567 was not associated with susceptibility to CHB, LC and HCC in people under the age of 50 and over 50.6. When stratified by drinking, we found no association between CR1 gene polymorphisms and CHB, LC, HCC susceptibility in drinker and non-drinker (P>0.05).7. When stratified by smoking, we found no association between CR1 gene polymorphisms and CHB, LC, HCC susceptibility in smoker and non-smoker (P>0.05).8. When stratified by race, we found no association between CR1 gene polymorphisms and CHB, LC, HCC susceptibility in Zhuang and Han population (P>0.05).9. After conducted the haplotypes between positions rs3811381 and rs2274567 of CR1, the differences of AC and GG haplotypes were not statistical significant btween control group and case group(P>0.05).Conclusion1. The rs3811381 polymorphism of G allele may be risk factors for LC in Guangxi female.2. The rs2274567 polymorphism of GG genotype may be risk factors for HCC in Guangxi male.3. The rs3811381 polymorphism of GG genotype may be risk factors for HCC. in people over 50.4. The rs3811381 polymorphism of CG genotype and G allele may be protective factors for HCC in people under the age of 50.