| Background and objective:Alzheimer's diaease(AD) is a neurodegenerative disease with progressively deteriorated memory and cognition. The main pathological features are the presenceβ-amyloid protein in senile plaques and neurofibrillary tangles composed of intraneuronal abnormal hyperphosphorylation of tau which accumulates into double-helical filaments (PHF). Although the exact pathogenesis of Alzheimer's disease has not yet been clarified, recent studies have shown that PLA2 mediateβ-amyloid protein deposition, synaptic loss, oxidative stress and inflammatory response, and pathogenesis. It is closely related with pathogenesis of multiple aspects of AD.and plays an important role in the occurrence of AD.Also there were some studies found that cytosolic phospholipase A2 involved in neuronal toxicity of Aβ.Overseas studies have found that cytosolic phospholipase A2 (cPLA2, PLA2G4A) gene polymorphism is correlated with AD, suggesting that cPLA2 and its gene polymorphism in the pathogenesis of AD may play an important role. There has not any relevant research reported about Chinese yet. This study was to evaluate the relationship between PLA2G4A BanI, PLA2G4B BamHI polymorphisms and susceptibility to Alzheimer's diaease in Hunan Han population, explore genetic background of Alzheimer's disease and pathogenesis, as Gene diagnosis and treatment of AD to provide a theoretical basis.Method:we perfomed case-control study to analyze the distribution and interaction of PLA2G4A BanI and PLA2G4B BamHI allele, genotype and interaction in Hunan Han population of China comprising 158 AD patients and 126 healthy controls by polymerase chain reaction(PCR) and restriction fragment length polymorphisms(RFLP).Results:1.The frequencies of AA,GA,GG genotype of PLA2G4A BanI were 12.0%,38.6%,49.4% respectively among AD group, and 10.3%,36.5%,53.2% respectively among non-dementia controls. The genotypes between the AD group and the control group have no significantly difference in the distribution (P>0.05). The frequencies of A,G allele were31.3%,68.7% respectively among AD group, and 28.6%,71.4% respectively among non-dementia controls. Allele analysis showed the alleles between the AD group and the control group have no significantly difference in the distribution (OR=1.141, P>0.05).2.The frequencies of AA,AG,GG genotype of PLA2G4B BamHI were 56.3%,34.2%,9.5% respectively among AD group, and 18.3%,42.1%,39.7% respectively among non-dementia controls. AA genotype in the AD group are significantly higher than in the control (P<0.05), which suggested that those who bring AA genotype have a higher risk of AD disease. The frequencies of A,G allele were 73.4%,26.6% respectively among AD group, and 39.3%,60.7% respectively among non-dementia controls. Allele analysis showed the A allele was significantly higher the the control(OR=4.268,P<0.05), suggesting that those carrying A allele may have a higher risk of AD.3. The incidence rate of BamHI AG+AA and BamHI GG carriers had significant difference in BanI GG and BanI GA+AA subgroup (P<0.05 (χ2=34.457, P=0.000)); The incidence rate of BamHI AG+AA and BamHI GG carriers had significant difference in BanI GA+AA sugroup (P<0.05 (χ2=27.415, P=0.000))Conclusion:1. There were no significant association between PLA2G4A BanI polymorphism and AD in Hunan Han population of China.2. AA genotype and A allele in the PLA2G4B BamHI may be associated with Hunan Han population of China in the pathogenesis of AD.3. PLA2G4A BanI and PLA2G4B BamHI polymorphism have a possible interaction in their effects on the presence of AD. |
