The Changes And Related Research Between Serum IL-17 And IgE In Mice With Bronchial Asthma

Objective:1. Establish mice model of asthma by OVA injection and sensitization. 2. Observe the changes of cell classification in bronchoaleolar laage (BLAF) and pulmonary histopathologically in the mice with the bronchial asthma in acute, chronic, remission period and the normal mice. 3. Observe the changes of serum IL-17 and IgE level in the mice with the bronchial asthma in acute, chronic, remission period and the normal mice. 4. To investigate the role and correlation of IL-17 and IgE in the bronchial asthma immunology pathogenesis.Methods: 40 kunming mice which 4 ~ 6weeks old, 18 ~ 22g health, female, clean level, were randomly divide into acute asthma group A1, chronic asthma group A2, asthma alleviate group A3 and normal control group C, to establish asthma model with the method of egg albumin(OVA) sensitization and stimulate: A1, A2, A3 group in the first 0, 7, 14 days with 20μgOVA+150μlAI(OH)350μlNS(100mg/L) intraperitoneal injection and sensitization; The first 28, 29, 30 days will be every mice of A1, A2, A3 group placed in 5L separate airtight container, to aerosol inhalation of 2% OVA, make mice exposed to OVA smog 20~30mins until appear in asthma attacks; Executed A1 group in the first 30 days 24 hours later after atomization; A2 group aerosol inhalation of 2% OVA every day in the 31 ~ 69 days; A3 group after 30 days don't treatment any more; In group C used normal saline intraperitoneal injection and aerosol inhalation, treatment time was the same to A2 group; The first 70 days executed A2, A3, C group. After taking 1.5 ml blood from eyeball, executed by cervical dislocation, centrifugal, take the upper serum, detect serum levels of interleukin-17 (IL-17) and IgE by enzyme-linked immunosorbent assay; Count cell classification in BLAF; take lung tissue, observation inflammatory cells infiltrating by HE staining. The obtained empirical datum uses the SPSS 18.0 Software to carry on the analysis. Results:1. The performance and comparison of asthma mice model: the mice of A1, A2, A3 group both apparent restlessness, easily frightened, shortness of breath, wipe nasal, sneezing, lip tail purple cyanosis, activity frequently, fur shadowbane, abdominal muscle spasm, incontinence, etc; A2 group showed more especially noticeable, the rhythm of breathing not neat, fell motionless, limbs undermines. The symptoms of A3 group are obvious early, alleviate later. C group did not appear afore-mentioned symptoms.2. Serum IL-17 level: A1 group for 15.14±1.98ng/ml, A2 group for 20.63±1.90ng/ml, A3 group for 10.96±1.44 ng/ml, C group for 0.39±0.46 ng/ml. A2 group higher than A1, A3 and C group (P<0.01), the difference was statistically significant; A1 group higher than A3, C group (P<0.01), the difference was statistically significant; A3 group higher than C group (P<0.01), the difference was statistically significant.3. Serum IgE level: A1 group for 6.63±0.78 ng/ml, A2 group for 6.94±0.12 ng/ml, A3 group for 6.22±0.15 ng/ml, C group for 5.44±0.24ng/ml. A2 group higher than A1, A3 and C group (P <0.01), the difference was statistically significant; A1 group higher than A3, C group (P<0.01), the difference was statistically significant; A3 group higher than C group (P<0.01), the difference was statistically significant.4. BALF cell aggregates and EOS%, NE%: A2 higher than A1, A3 and C group (P<0.01), the difference was statistically significant; A1 group higher than A3, C group (P<0.01), the difference was statistically significant; A3 group higher than C group (P<0.01), the difference was statistically significant.5. Serum IL-17 and IgE level positively correlated (r=0.942, P<0.01), the difference was statistically significant; Serum IL-17 and EOS% of BALF positively correlated ( r=0.956, P < 0.01) , the difference was statistically significant; Serum IL-17 and NE% of BALF positively correlated (r=0.938, P<0.01), the difference was statistically significant.6. Pulmonary histopathologically observation: A1, A2, A3 group compared with C group showed lung surface swelling, increase in size, cut-side visible oozy white foam. Lung biopsy HE dyeing appear bronchial lumen narrow, mucosa edema, mucous glandular hyperplasia, mucous membrane, the alveolar every thickening, edema irregular shape, the alveolar space fusion expanded. A1 group bronchial mucosa and the submucosa, smooth muscle and perivascular note the amount of inflammatory cells infiltrating; A3 group inflammatory cells infiltrating slightly reduced; A2 group is based in the acute phase of pathology appear on airway epithelial fibrosis and vascular scar, appear the airway remodelling.C group mice trachea and alveolar structure normal, bronchial epithelium complete, did not see the obvious inflammatory cells.Conclusion:1. Successful establish the model of mice in bronchus asthma, pulmonary histopathologically in the mice with the bronchial asthma acute, remission, chronic period and the normal contol mice accord with the pathophysiological changes in asthma.2. IL-17 in asthma immunological pathogenesis plays stimulative effect, participate in the acute period of asthma inflammation and airway remodeling processes, and the level of IL-17 to a certain extent said the severity of asthma.3. Asthma mice immune function disorder, existing IgE, EOS, NE in the acute period increased, chronic period increased significantly, and remission period slightly reduced, but still more higher than normal level, explain IgE, EOS, NE both participated in asthma events.4. The serum IL-17 and IgE level of bronchial asthma mice is positive correlation, jointly promote the development and happening of asthma.