| ObjectiveThrough the D-gal combined with the drug scopolamine, sodium nitrite, ethanol celiac injections copy subacute aging AD model rats, observe the model of rats related aging signs and behavior performance, detection this model in rats SOD,MAO and the content of MDA, built with energy level, evaluation recovery Dan flavored the brain party of the influence of free radicals and memory effects.Methods1. Three months health SD rats, there are half-half the males and females.84 evenly divided into seven groups at random, each group, divided into 12 only control group (blank group, the model group, physiological saline group), the treatment group (recovery Dan flavored party group, recovery Dan group, phlegm, and group, brain health group). Gal, intraperitoneal injection D-and in different time intraperitoneal injection the drug scopolamine, sodium nitrite, ethanol, interfere with the memory and the extraction of representation function; Morris water maze testing with the ability to learn and memory function. 2. Morris water maze experiment ends, executed rats, instant remove its brain hippocampal organization, and the organization made 10% serum (seahorse:organization.1:9), buffer liquid= groups then determined brain SOD,MAO,MDA, the activity level built.Results1. from the space exploration experiment and orientation navigation experiments to see, D-gal combining different time in the drug scopolamine, sodium nitrite, ethanol celiac injections copy subacute aging AD model, the method of rats can be effectively led to the experimental rats learning ability and memory dysfunction.2. Compare the average escape experimental rats, the average sports distance, incubation period across platform frequency. In the control group, the blank group within with model group, compared to normal saline groups was significant difference (P < 0.05 & P< 0.01), has a statistically significant; Saline groups and model group no significant differences, no statistically significant (P> 0.05). In comparison between groups, recovery Dan flavored with blank group, fang group with brain khalaf Dan group rehabilitation group, no significant difference was not statistically significant (P> 0.05); Recovery Dan group, brain health group, phlegm, and groups and model group, physiological saline groups are compared, significant difference (P< 0.05), a statistically significance; Eliminating phlegm to group and recovery Dan group, brain, rehabilitation group compared was significant difference (P< 0.05), has a statistical significance; Recovery Dan group and brain no significant difference, rehabilitation group was not statistically significant (P> 0.05).3. About group experimental rat brain SOD, MDA, built level of tests showed that the level of SOD, improve, inhibition of activity built, MDA, recovery Dan flavored square with model group, physiological saline, group compared with significant difference (P< 0.05 & P< 0.01) and statistical significance; Compared with the blank group, there is no significant difference (P> 0.05), no statistical significance; Recovery Dan flavored square with recovery Dan group, phlegm, and group, brain health group compared, which has obvious difference (P< 0.05 & P< 0.01) and statistical significance.Conclusions:1. The spleen qi deficiency, weak, kidney sputum MengQing qiao is fundamental pathological alzheimer's disease pathogenesis, spleen and kidney double compensation and consoliated cost the school, phlegm, and begin to understand it can effectively improve AD model rat study ability and memory function.2. Recovery Dan flavored by spleen and kidney double fill party school, phlegm, and begin to understand and consolidate effectively improve brain SOD, inhibit the activity, built and MDA antagonism of free radicals on the neurons, protecting neurons damage with physiological function, learning ability and prevention and control the effect of impairment of memory.Subject wordsstrengthening the impetus of Body, nurturing primordial Qi and promoting brainpower; AD; OFR... |
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