The Significance And The Expression Of The Levels Of IL-13 And Eosinophil Count And IgE In Asthma Children Infected With Mycoplasma

Objective: 1.To detected the expression of the levels of IL-13 in asthma children infected with mycoplasma and not infected;2. To detected the expression of the levels of Eosinophil count (EOS) in asthma children infected with mycoplasma and not infected;3.To detected the expression of the levels of IgE in asthma children infected with mycoplasma and not infected;4.Discussed the correlation between mycoplasma infection and bronchus asthma.Methods: Choose the asthma patients 60 cases in dalian medical university affiliated first hospital outpatient department from May to September in 2010. Divide into 26 asthma patients with mycoplasma infection (infection group) , 34 patients with not mycoplasma infection (not infection group) . The levels of IL-13 were measured with ELISA and Peripheral Eosinophil count with Conventional classification and the levels of IgE were measured radio immunoassay (RIA) in 26 asthma patients with mycoplasma infection (infection group) , 34 patients with not mycoplasma infection (not infection group) , and 25 healthy children ( normal control group) . experimental data Mean differences standard deviation.Use the SPSS13.0 software to analysis the experimental data. Mean between the two groups were compared by chi-square test.Association between groups was analyzed by Single factor analysis of variance ,with P<0.05 as statistically significant.Results:1.The levels of IL-13 The levels of IL-13 in infection group is 196.64±19.153pg/ml, and in not infection group is 145.91±14.273pg/ml, the levels of IL-13 in control group is 79.00±10.909pg/ml, the levels of IL-13 were significantly higher in infection group than in no infection group (P<0.05) , in infection and not infection group were significantly higher than control group (P< 0.05) .2.The levels of EOS The levels of EOS in infection group is 617.68±27.759×106/L, and in not infection group is 453.24±21.120×106/L, the levels of EOS in control group is 132.00±53.855×106/L, the levels of EOS were significantly higher in infection group than in no infection group (P<0.05) , in infection and not infection group were significantly higher than control group (P< 0.05) .3.The levels of IgE The levels of IgE in infection group is 638.92±104.674IU/ml, and in not infection group is 313.97±80.171IU/ml, the levels of IgE in control group is 64.05±22.766IU/ml, the levels of IgE were significantly higher in infection group than in no infection group (P<0.05) , in infection and not infection group were significantly higher than control group (P< 0.05) .4.The relation of IL-13 and IgE The relation of IL-13 and IgE in infection group is positive( r = 0.961 , P<0.05) , The relation of IL-13 and IgE in infection group is positive( r = 0.949 , P<0.05) .Conclusion:1.The levels of IL-13 were significantly higher in asthma children infected with mycoplasma than in asthma children not infected with mycoplasma and control group. Prompting that cytokines will express abnormal when organism infected with mycoplasma.2.The levels of IL-13 were significantly higher in asthma children infected with mycoplasma than in asthma children not infected with mycoplasma and control group. Prompting that organism exist allergy inflammation when organism infected with mycoplasma.3. The levels of IgE were significantly higher in infection group than in no infection group and control group. Prompting that the levels of IgE maybe related to allergen irritated repeatedly. When organism infected with mycoplasma, mycoplasma be acted specific allergen irritated organism produce IgE, result in bronchus asthma attacked.4.Mycoplasma infection is related to bronchus asthma.