Study On The Extraction,Oxidative Decomposition And Anti-Tumor Activities In Vitro Of Volatile Oil From Atractylodes Macrocephala Koidz.

Volatile oil is the main active ingredient of the Atractylodes macrocephala Koidz. Because of its exact antitumor effects and small side effects, it has a good prospect in antitumor drugs. However, the unstableness and easy oxidation make the drug discovery difficult. In response to these, the approach we are taking to solve the stability problem of volatile oil is to let it go through quick oxidation and decomposition, turning into volatile oil with anti-tumor effects. The specific contents of the research are as follows.1. It was found that the best extraction solvent of Volatile oil is ethyl acetate (2.56%) and the best extraction methods is supercritical CO2 extraction (2.76%). The optimum conditions of oxidation and decomposition is outdoor light (19-27℃), The decomposition rate of atractylone is 98.08%.2. Five anti-tumor components of Volatile Oil before and after oxidative decomposition from Atractylodes macrocephala Koidz were determinated by HPLC. The results showed that atractylone andγ-elemene content was zero after oxidation, the other three components were significantly increased,β-eudesmol content in Volatile Oil by supercritical CO2 extraction was the highest from Anhui,1527.56 times higher than that before oxidative decomposition. AtractylenolideⅠ,Ⅲcontent was the highest by steam distillation from Hebei and 4.30 times higher than that before oxidation.3. GC-MS were employed to analsis ingredients and contents of Volatile Oil before and after oxidative from Atractylodes macrocephala Koidz with different method. The results show that the main anti-tumor component of Volatile Oil after oxidative decomposition, the content of atractylone andγ-elemene was decreased by 95% and 98%, while the increment of atractylenolideⅠ, atractylenolideⅢ, costunolide,β-eudesmene and Isovelleral is 3.68-11.04 times more than that before oxidation Volatile Oil.4. Content of 10 kinds of trace elements(Mg,Al,Fe,Cr,Cu,Mn,Ni,Sr,Ti,Zn)in Atractylodes macrocephala Koidz, volatile oils and volatile oil of Oxidative decomposition was determined by ICP-AES. The results indicate that there existed greatly difference in different extraction conditions.5. This experiment researched the effect on the ovarian cancer cell SKOV-3 of Volatile Oil before and after oxidative decomposition from Atractylodes macrocephala Koidz. The volatile oil has the function of proliferation inhibition of ovarian cancer cell, and this effect is in dose and time dependent manner. Affer dealing with the concentrations of 200mg/L, it had highest inhibition rate,and it were significant after 72 hours at different concentrations. Effects on skov-3 apoptosis and cell cycle induced by volatile oil of atractylodes macrocephala koidz before and after oxidative decomposition was studied by Flow Cytometry. Affer dealing with the concentrations of 200mg/L it had highest apoptosis rate, a cell cycle-specific in G2/M and S phase, respectively.