Association Of ABCA1 Gene Polymorphism And Risk Factors With Cerebral Infarction

Objective:To investigate the ATP binding transporter-1 (ABCA1) gene polymorphisms in the progress of cerebral infarction, and explore the influence of the ABCA1 gene polymorphisms to the pathogenesis of cerebral infarction.Methods:240 patients with cerebral infarction and 240 health controls were collected, and DNA were extracted with non-spin column. The ABCA1 gene SNP C69T, R219K, 6645+55 Gâ†'C genotype were detected by using TaqMan-PCR and allele frequencies were. calculated.The glucose,lipid, blood uric acid of the serum were measured, and the risk factors and General clinical data were compared between cases and controls. Then to further explore the cerebral infarction subgroup with the genotype and allele frequency which were statistically significant in the two groups, and build Logistic regression analysis model.Results:1,ABCA1 C69T Loci:Compared with the controls(47.9%; 43.3%; 8.8%), the CC,CT,TT genotypes distribution(45.8%; 44.6%; 9.6%) in cerebral infarction group, have no significant difference(χ2=0.209,0.076,0.100;P=0.647,0.783,0.752).And allele frequencies also have no significant difference(χ2=0.238,P=0.626).2,ABCA1 G596A Loci:Compared with the controls(18.8%; 45.4%; 35.8%), the GG,GA,AA genotypes distribution(15.0%; 40.4%; 44.6%) in cerebral infarction group, have no significant difference(χ=1.203,1.225,3.822;P=0.273,0.268,0.051).And allele frequencies also have no significantly difference(χ2=0.238,P=0.626).A allele frequency of cerebral infarction (0.648)was higher than control group (0.585,χ2=3.966, P=0.046), allele A can increase the risk of cerebral infarction (OR=1.303,95% CI= 1.004-1.692).3,ABCA16645+55Gâ†'C Loci: The difference of GG genotype distribution between the control group(2.5%) and the cerebral infarction group(7.5%) was statistically significant (χ2=6.316, P=0.012); GG homozygous genes type may increase the risk of cerebral infarction (OR= 3.162,95% CI =1.233-8.111), G allele frequency in cerebral infarction group (0.290)was higher than the controls (0.200,χ2=10.418, P=0.001), allele G may increase the risk of cerebral infarction (OR=1.630,95% CI=1.210-2.197).Cerebral infarction group CC genotype (49.6%) distribution compared with control group (62.5%). the difference was statistically significant (x2=8.127, P=0.004):Carry CC homozygous genes type may increase the risk of cerebral infarction (OR=3.162, 95%CI=1.233-8.111), C allele frequency in cerebral infarction group (0.290) was lower than the controls (0.800,χ2=10.418,P=0.001), allele C may reduce the risk of cerebral infarction (OR=0.613,95% CI=0.455-0.826)Genotype (χ2=12.758, P= 0.002) and allele frequency (χ2=5.557, P=0.018) were statistically significant differences betwen artery thrombosis cerebral infarction group and the controls. It indicate 6645+55 Gâ†'C (G/C) primarily associated with arterial thrombosis cerebral infarction.ABCA16645＋55 Gâ†'C non-conditional Logistic regression analysis showed that GG genotype to the risk of cerebral infarction was 17.7 times of other risk factors (P 0.020, OR=17.689).4. Serum GLU,TC,TG,LDL-C,UA,Hct,LP (a) but HDL-C and ApoAl were significantly higher in cerebral infarction group than the controls,Difference was statistically significant(p＜0.05). Apolipoprotein B100 (ApoB100) levels were lower and the difference were not statistically significant (P>0.05). Family history, history of smoking, drinking histories, diabetes history, history of hypertension incidence and BMI, SBP.DBP with cerebral infarction were higher than the control group, the difference were statistically significant (P< 0.05), age and gender were not statistically significant (P> 0.05).Conclusion:1,There are CC/CT/TT polymorphism of ABCA1 C69T,GG/GA/AA polymorphism of ABCA1 G596A, GG/GC/CC polymorphism of ABCA16645+55 Gâ†'C.2,A allele frequency is higher than the controls of ABCA1 G596A, A allele may be a risk factor for cerebral infarction.3,ABCA16645＋55 Gâ†'C GG genotype and G allele frequency are higher than control group. Non-conditional Logistic regression analysis showed that the GG genotype and G allele are likely to be a risk factor for cerebral infarction.4,G allele frequency of arterial thrombotic cerebral infarction sub-group is higher than the control. It indicated 6645＋55 Gâ†'C (G/C) primarily associated with arterial thrombosis cerebral infarction.5,The cerebral infarction may be induce by the increased levels of serum GLU,TC,TG,LDL-C,UA,Hct,LP (a).The increased levels of HDL-C,ApoA1 may reduce the incidence of cerebral infarction.6,Family history,smoking history,drinking history,history of diabetes,hypertension,body mass index,systolic and diastolic blood pressure are probably related risk factors for cerebral infarction.