| Objective To study the effects of sleeve gastrectomy on food intake, body weight, GLU, serum lipid and gastrointestinal hormone in Goto-Kakizaki rats.Method Thirty male GK rats were randomly divided into three average groups, SG group with nine animals,Sham SG group with seven animals,Pair food group with seven animals. SG group was treated with sleeve gastrectomy. The Sham group only had stomach section and then sutured, Stomach tissue is not removed. Pair food group was treated with daily quantitative diet. Continuous monitoring the food intake and body weight in each group. Taking blood 1d pre-operation, 2w, 4w, 6w, 8w, 10w, 14w, 24w post-operation respectively. Then centrifuge the blood and reserve the upper serum. Determined on fasting blood glucose and blood lipid, ELISA was used to test the plasma levels of insulin and gastrointestinal hormone. OGTT was used to test the regulation of blood sugar. Result1. Food intake: Postoperation the SG group have an obvious smaller amount of food intake in 24 weeks(P<0.01). The food intake of the sham group was notable higher than the SG group(P<0.01). The food intake of the pair food group as same as postoperation of the SG group(20.8±1.9g).2. Body weight: The 3 groups have a weight loss until to week 2, and then a progressive increase steadily of weight was observed from week 2 to week 24. the average weight of SG group was lower than the sham group in the period from week 4 to week 24(P<0.01), while the pair food group have no difference(p>0.05).3. FBG: Postoperation the SG group have an obvious low level of fasting blood glucose in the period of 24 weeks(p<0.05), while the Sham group and the pair food group have no difference. the level of FBG in SG group was lower than PF group and Sham group(P<0.01).4. OGTT: glucose tolerance of the SG group was observed a remarkable improvement than the time of preoperation(P<0.01), while the Sham group and the pair food group have no difference(p>0.05). the AUC in 2nd week decreased by 28.1%(P<0.01), the level of blood glucose of the SG group after 2 week is obvious lower than PF group and Sham group(P<0.01), and the AUC of SG group is decreased by 21%(P<0.01), Compared with the Sham group and the pair food group.5. FISN: Serum insulin level of the 3 groups presented no remarkable change in 24 weeks(p>0.05).6.Ghrelin: Postoperation the SG group have an obvious reduce of the level of ghrelin(P<0.01), while the Sham group have no significant difference(p>0.05). Postoperation the level of ghrelin in SG group was obvious lower than Sham group(P<0.01),while the SG group and the Sham group have no significant difference pre-operation (p>0.05).7. GLP-1: The GLP-1 level of the SG group presented a continually high state and was significantly higher than the Sham group in 24 weeks(P <0.01). Postoperation the Sham group have no remarkable change (p>0.05).8. TG, TC, HDL: 10 weeks after operation, The TG and TC level of the SG group was obvious lower than Sham group(P<0.01), and The HDL level of the SG group was obvious higher than Sham group(P<0.01).Conclusion1. In this study, we investigated the effect of sleeve gastrectomy in non-obese type 2 diabetic rats. Postoperation of the SG, the weight of the rats increased steadily after 2 weeks, The results indicated that Sleeve gastrectomy will not affect the normal weight in non-obese type 2 diabetic rats, and there is a increase steadily of weight.2. After operation of SG, fasting blood glucose was significant reduced, and glucose tolerance of the SG group was observed a remarkable improvement, The study findings suggest that the therapeutic effect of SG in non-obese T2DM is not depend on weight and food intake, there are some other mechanism.3. Serum ghrelin were significantly reduced and Serum GLP - 1 were significantly increased, These indicating that the sleever gastrectomy affected these gastrointestinal hormone levels, and the change of these gastrointestinal hormone has certain relationships with blood glucose. sleever gastrectomy may be Play the role of controlling blood glucose by affecting these gastrointestinal hormone.4. lipid metabolism can be also improved by SG, and SG can reduce risk factors of cardioascular disease, this study provides certain experimental basis for the surgical treatment of type 2 diabetes and dysmetabolic syndrome. |
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