Study On PROS1 Pro626A/G Polymorphism In Uygurs And Hans In Pulmonary Thromboembolism

Objectives: To study the difference in the factor of pulmonary thromboembolism patients in the Uygur and the Han nationality by PROS1 Pro626A/G PolymorphismMethods:Collection of specimens according to the inclusion criteria (total included 100 patients,100 healthy controls), of which 50 cases of patients Han, Uygur patients with 50 cases,50 cases of healthy controls Han, Uygur and healthy control group of 50 patients.Of whole blood samples taken from extracted DNA, according to SNP sequence information attachment sites, the use of MassARRAY technology platform designed primers to complete the sample preparation, PCR, SAP, single base extension, point-like mass spectrometry on PROS1 Pro626A/G and more state of genetic polymorphism.SPSS16.0 software used for statistical analysis, genotype and allele frequencies compared by x2 test, and calculate the associated risk value (OR).Results:1-100 cases of PTE in addition to 10 cases of secondary risk factors is unknown, the rest have one or several secondary risk factors coexi.2.A, AG and G genes also appearing at the 626th sequencing site of PS gene poline,confirmed that on this site,AA,AG and GG alleles existing,and the genotype distributions fit the balance law of Hardy-weinbery.3. The overall case group and control group AA, AG, GG genotype frequency distribution of the three statistically significant difference,x2=11.527, P=0.003. G allele in the case group and control group, frequencies were 0.90,0.785, the two groups x2=9.967, P=0.002.Relatire to the genotype of AA and AA+AG,OR of the mutated homozygote GG genotype who happened to PTE respectively is 0.364,0.338, P is 0.395,0.001.4.The differences of frequency of AA,AG and GG of case group and significant(x2=9.416,P=0.009),distribution frequenly of case group and control group of allele G respectively is 90% and 76%,the difference is statistically significant(x2=6.945,P=0.008).Compared with who carried AA and AA+AG,OR of homozygous GG who carrying mutations happened to PTE respectively is 0.659 and 0.258,P is 0.770,0.003.5.The differences of frequency of AA,AG and GG of case groups and control group of han are unstatistically significant (x2=3.704,P=0.157),distribution frequency of case group and control group of allele G respectively is 90% and 81%,the difference is unstatistically significant(x2=3.267,P=0.071).Relative to the genotype of AAand AA+AG,the OR of the mutated homozygote GG genotype who happened to PTE respectively is 2.250,0.444,P is 0.268,0.075.6.The difference is unstatistically significant. Compared uighure and han,the difference of frequency distributions of AA,AG and GG is unstatistically(x2=1.235,P=0.539).Compared with who carried AA and AA+AG,OR of homozygote GG who carrying mutation happened to PTE respectively is 1.976,0.878,P is 0.326,0.799,the difference is unstatistically significant.Conclusion:1. Uygur and Han there PROS1 Pro626A/G polymorphism.2. PROS1 Pro626A/G polymorphism with Uighur pathogenesis of PTE, but the GG genotype homozygous mutation failed to increase the occurrence of PTE Uygur risk of.3.PROS1 Pro626A/G polymorphism may be related to PTE in Chinese Han, the need to increase sample size to further validate.4.Uygur and Han pulmonary thromboembolism PROS1 Pro626A/G polymorphism may be different, yet to increase the sample size is further confirmed.