Explore The Performance And Mechanism Of Erythrocytes Oxidative Damages Based On Mice Exhaustive Swimming

Erythrocytes oxidative damages induced by exercise are very concerned in sports medicine. They could aggravate tissue hypoxia during exercises, effect motor capacity and restoration process of individuals; Erythrocytes oxidative damages are also the considerable reasons of sports anemia. Although there are some ways of administering antoxidants to reduce these damages, the mechanism of erythrocytes oxidative damages induced by exercises is not fully clear. This research is to explore the performances and restoration process of erythrocytes oxidative damages with mice exhaustive swimming, and the effect of blood oxygen content on the damages above.Method: Forty male 8-week-old Kunming mice were randomly divided into sedentary control, single bout of exhausted swimming, exhausted swimming with 1 hour recess, exhausted swimming with 12h recess and exhausted swimming with 24h recess groups. Took blood samples with pick eyeballs, measure P50, ferrihemoglobin(MetHb), reduced glutathione hormone (GSH), membrane lipid peroxidation (thiobarbituric acid–reactive substances,TBARS), sulfhydryl (SH) level of erythrocytes plasma membranes, and analyzed changes of membrane proteins with SDS-PAGE. To explore the effect of blood oxygen content on erythrocytes oxidative damages, keeping oxygen saturation(SO2)of cultured RBC respectively at the states of 0.3, 0.5, 0.7, 0.9and 0.98 (3% RBC hematocrit)in vitro, oxidative stress was induced by tert-buthylhydroperoxide (BHP, 0.15mmol/L of final concentration).Results: (1) P50 and MetHb were obviously increased immediately and 1h postexercise(P<0.01;P<0.05), they recovered normal 12h postexercise; GSH and SH remarkably decreaced immediately and 1h postexercise(P<0.01;P<0.05), and recovered normal 12h postexercise; TBARS increased immediately and 1h postexercise(P<0.01), it remain higher 24 h postexercise than normal(P<0.05); A high molecular weight membrane protein band(HMB) which was mainly composed of Spectrin and Band 3 linked with disulfide bond, was detected immediately and 1h postexercise, it losted 12 postexercise. (2) The experiment in vitro showed that the in the presence of BHP, erythrocytes antioxidant capacity increased from 0.3 to 0.98 groups, while oxidative damages decreased with the rise of SO2.Conclusion: Exhaustive swimming can induce erythrocytes oxidative damages, although some damages recovered to normal after exercise, membrane lipid peroxidation hard to restore, this change could increase erythrocytes aging and scavenge. The erythrocytes antioxidant capacity and oxidative damages are closely related to blood oxygen content, and declined oxygen content could promote erythrocytes oxidative damages. Considering exhaustive exercise along with the decline of venous oxygen content, this decline could promote erythrocytes oxidative damages.