Research On Antioxidant Activities Of Pleurotus Citrinopileatus And The Anti-Fatigue Effects Of Pleurotus Citrinopileatus Beverage

Objective:To compare the antioxidant effects of Pleurotus citrinopileatus in fresh and dried and the anti-fatigue effects and the mechanism of Pleurotus citrinopileatus beverage.Methods:①The antioxidant effects of Pleurotus citrinopileatus extracts were determined through the orthogonal experiment in which DPPH·scavenging capacity for evaluation. Moreover, determined the scavenging capacity at different concentrations of P. citrinopileatus extracts to the DPPH·,·OH, H2O2, the antioxidant capacity of P.citrinopileatus extracts were analyzed.②P. citrinopileatus were used as main raw material to develope health drinks. Mice were randomly divided into a control, a positive control and three p. citrinopileatus beverage groups, each of 10. They were orally administered with distilled water (for control), the Red Bull positive control and three different doses of P. citrinopileatus juice (83,165 and 330 g·L-1,0.1 mL·10g-1·BW, respectively) for 30 days. There were to pole climbing test in the 10th,20th and 30th days. Then record the pole climbing time. Weighed, thymus index and spleen index were detected after climbing pole experiment in the 30th day.③In the anti-fatigue test, group the mice into a control, a positive control and three P. citrinopileatus beverage group. The blank group were ig with water, the positive control were ig the Red Bull, three control group were ig with P. citrinopileatus juice of different concentrations (83,165 and 330 g·L-1,0.1 mL·10 g-·BW) for 30 days. Then record the exhaustive swimming time. In an exhaustive swimming task, the lactic acid levels, blood urea nitrogen (BUN) levels, superoxide dismutase (SOD), maleic dialdehyde (MDA) levels of serum of liver, the carbonyls and thiol content of proteind, liver and muscle glycogen stores of the mice were tested.Results:①The method of orthogonal experiment which determined the antioxidant effects of P. citrinopileatus extracts was as follows:The dried P. citrinopileatus extracted with methanol at 20℃for 30 min, while the fresh P. citrinopileatus were extracted with distilled water at 50℃for 60min. The antioxidant activity of P. citrinopileatus extraction is higher in fresh mushroom than dried chacter. Dried and fresh products of P. citrinopileatus are both have the ability to scavenge DPPH·,·OH and H2O2, and the scavenging activity enhanced with increasing concentration, especially to the H2O2, they showed strong scavenging activity. For the DPPH·,·OH and H2O2 clearance rate, the extracts of fresh P. citrinopileatus higher than the dried one.②The climbing pole times of P. citrinopileatus beverage groups were longer than the control and the positive control group (P<0.05) and climbing pole times were longer with the P. citrinopileatus concentration increases. The extensive times percentage of climbing pole on P. citrinopileatus beverage groups have also increased as the extend of intragastricing times. P. citrinopileatus beverage has no effect on thymus index, spleen index and body weight in mice.③Different doses of P. citrinopileatus juice beverage can prolong exhaustive swimming time (P<0.05 or 0.01), reduced BUN levels (P<0.05 or 0.01 or 0.001), decreased accumulation of lactic acid in the blood (P<0.05 or 0.01 or 0.001) increased SOD and lower MDA content of serum (P<0.01 or 0.001) and increased protein-bound thiols content and lower protein-bound carbonyls content of liver (P<0.001), and increased liver and muscle glycogen levels (P<0.01 or 0.001)Conclusion:Based on the results, P. citrinopileatus extraction showed strong antioxidant effect, and the fresh is stronger than the dried chacter. The P. citrinopileatus beverage showed obvious anti-fatigue effect in mice.