| Pleurotus citrinopileatus is belong to Basidiomycotina Hymenomycetes class Agaricales Pleurotus is one kind of precious economical fungus,People often edible, the taste is beautiful,fragrant,has certain healthcare function.This year studies indicated that Pleurotus citrinopileatus mainly includes fatty acid ingredients and so on sterol class.But present is not comprehensive to the Pleurotus citrinopileatus effective component's research.The Pharmacology research also just started.The author through the silica gel column chromatographic analysis,separation methods and so on gelatin column chromatographic analysis separates the monomer compound from Pleurotus citrinopileatus,Through physical and chemical properties,spectrum of modern methods and techniques(ESI-MS,1HNMR,13CNMR),Determined that their structure respectively is mannitol,ergosterol,ergotsa-7,22-dien-3β,5α,6β-triol,adenosine,uracil, nicotinamide,inosine,guanosine.The mannitol carries on the quota using HPLC-MS/MS to Pleurotus citrinopileatus.Uses the multi-response examination with MS/MS as the HPLC detector (MRM) to scan the way,Has established in HPLC-MS/MS determination Pleurotus citrinopileatus the mannitol content method.This method's linearity range is 0.208-1.04mgmL-1.Through the sample Canadian sign experiment,the returns-ratio is 97.12%,accuracy RSD(n=5) is 0.94%.The actual sample analysis indicated that in Pleurotus citrinopileatus the mannitol content is 38.9mg/g.This method selectivity high, is keen,is fast.It also suitable in other complex systems the mannitol content fast determination. |
