| Background Renal cell carcinoma (renal cell carcinoma, RCC) is originated in the renal tubular epithelium of urinary system cancer, also called renal adenocarcinoma, referred to as kidney cancer. Renal cell carcinoma accounts for 80% -90% of Kidney malignant tumors, accounting for 2% -3% of adult malignant tumors, and with regional differences,Higher incidence in developed countries than developing countries,According to the Office of Cancer Research and the Ministry of Health survey showed:Malignant tumor of urinary tract showed an increasing trend in the incidence,male to female ratio is about 2:1, age of multiple age 50-70 and urban higher than rural.The present stage of the study show that RCC's etiology is not clearly.Currently available evidence suggests that The occurrence of RCC has relations with genetics, smoking, obesity, hypertension and antihypertensive treatment and so on.Cyclooxygenase (cyclooxygenase, COX), an important rate-limiting enzyme in prostaglandin (PGs) synthesis,here are two COX isoenzyme, COX-2 is one of them, is a membrane integrated protein that catalyzes the formation of arachidonic acid of prostaglandin H2. Studies have shown that the proliferation of tumor cells and metastasis were associated with prostaglandin synthesis, and prostaglandin synthesis was relation with regulation of COX-2,Sano H, et al found that COX-2 in peroxidase activity can catalyze the oxidation of a series of variants, COX-2 expression continued to inhibit apoptosis and inhibition of certain immune cells in the tumor may play an important role.Heparanase (heparanase, Hpa)) is an endo-glycosidase, is only found proteolytic enzymes to degrade the extracellular matrix and basement membrane HSPG, and it plays an key role in tumor cells throughing the vessel wall to achieve infiltration, proliferation. And its role received extensive attention. Proliferation and metastasis of tumors depend on angiogenesis, this study investigate that Hpa and COX-2's role in promoting tumor angiogenesis and metastasis and the relationship with prognosis.Objective To study protein expression of cyclooxygenase-2(Cox-2),heparanase(Hpa) and microvessel density(MVD) in the tissue of renal cell carcinoma(RCC), and investigate their clinicopathological and prognosis significance for RCC.Methods Immunohistochecal staining with StreptAvidin-Biotin Complex(SABC) was empoyed to determine the expression of Cox-2,Hpa and CD34 in RCC tissues from 60 cases and 30 of the nonnal renal tissue. Comparative analysis of Cox-2,Hpa expression and MVD in RCC tissue and the adjacent-tumor renal tissue.And analyse the relation between MVD expression and clinical pathology parameter,prognosis.Results Among 60cases of RCC tissues, the positive rate of Cox-2 and Hpa expression (X2=24.308,x2=20.089,P<0.05) were 71.7% and 63.3%,both of them were sig-nificantly higher than the nomal renal tissue.Increased expression of Cox-2 was associated with tumor size in RCC(x2=4.34, P<0.05). We did not observe relationship between Hpa expression and tumor size,but with lymphatic metastasis (x2=12.72, P<0.05).MVD for negative and positive Cox-2,Hpa expression were significantly different(P=0.001).MVD was related with histological grade, clinical stage, prognosis(P<0.05) of RCC.Conclusion The enhanced expression of Cox-2 was associated with tumor size; Cox-2 and Hpa can promote angiogenesis;MVD was relation with prognosis. |
