| ObjectiveSevere acute pancreatitis (SAP) is a fatal disease whose mortality is very high. The reason of the high mortality rate is mainly multiple organ failure and the subsequent infections, which due to intestinal mucosal barrier injury, intestinal bacteria and endotoxin translocation in SAP. This study is on the basis of rabbits' SAP models to detect the protective effect of somatostatin combined with growth hormone on intestinal mucosal barrier injury in SAP rabbits and the significance of the treatment of SAP.MethodsSeventy-two rabbits were random assigned into model group (SAP group), SS treated group (SS group) and SS combined with GH treated group (SS+GH group). SAP models were induced by retro-injection of 5% sodium taurocholate into the pancreatic duct. After modeling, all rabbits were given 5% glucose saline daily, The rabbits in SS group and SS+GH group were continuously given SS (3.5μg·kg-1·h-1) for 48 hours. Besides, the rabbits in SS+GH group were subcutaneously injected with 0.15IU/kg of GH at the 1st and the 24th hours after modeling. The levels of serum amylase, serum tumor necrosis factor-a (TNF-a) and plasma diamine oxidase were measured at the 6th,12th,24th and 48th hours after modeling. The pathological changes of pancreatic tissue and ileal mucosa were observed. Survival rate was calculated. Data were analyzed using SPSS 16.0 software. The univariate analysis was used to compare the difference among groups.Results1. The survival rate of the animals in every group:The survival rate of rabbits in SS+GH group was significantly higher than that of rabbits in SAP group and SS group.2. Serum amylase:Comparing with SAP group, the serum amylase of SS+GH and SS group decreased. The serum amylase of SS+GH and SS group is significantly lower than that in SAP group after 48 hours (P<0.05). There is no significant difference of serum amylase between SS+GH and SS group at each time point.3. Plasma diamine oxidase and serum TNF-a:Comparing with SAP group and SS group, the plasma diamine oxidase and serum TNF-a of SS+GH group decreased. The plasma diamine oxidase and serum TNF-a decreased significantly at 24 hours and 48 hours after disposal (P< 0.05).4. Pathological changes of pancreas and intestinal mucosa:Comparing with SAP group and SS group, the pathological changes of the animals of SS+GH group reduced markedly, and the pathology score was significantly lower (P<0.05). The inflammation of ileal mucosa was alleviated, and the integrity of bowel mucosa improved.Conclusions1. The intestinal barrier of SAP was damaged.2. SS can improve the intestinal mucosal microcirculation and maintain the intestinal mucosal barrier by decreasing the level of TNF-a.3. The treatment with SS and GH can improve intestinal mucosa barrier function, and significantly improve the prognosis of SAP in rabbits. |
