| Along with the economic development, people's life style has been changed dramatically and high energy intake is very common worldwide. As the consequence, the incidence of hyperuricemia and gout has been increased significantly which is believed as the risk factor of hypertension, cardiovascular disease and kidney failure. Dietary intervention for hyperuricemia control has attracted great attention.ObjectiveThe current study aims to investigate the effect of the Quercetin (Que), Apigenin (Api) and onion powder on the hyperuricemia in rats, to reveal the possible mechanisms by comparing the enzyme levels of xanthine oxidase (XOD), adenosine deaminase (ADA), superoxide dismutase (SOD) before and after the intervention.MethodsThere are three sessions of the study:Firstly, the suitable dosage level of Que on hyperuricemia in rats was determined in a pilot study. The hyperuricemic animal model was established by gavaging adenine and ethambutol to the SD rats. At the same time, the rats were given quercetin at different dosages. The effective parameters of quercetin on hyperuricemic animal were serum uric acid (SUA), blood urea nitrogen (BUN), serum Creatinine (SCr) and superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant capacity (T-AOC) and the activity changes of the XOD and ADA in serum and liver.Scondly, quercetin level in powder onion was quantified through HPLC assay.Thirdly, the effects of quercetin, apigenin and powder onion on hyperuricemia in rats were compared. Based on the result of the first section, Que, Api and powder onion were given to the hyperuricemic rats at the Que level of 10mg/kg per day.ResultsIn the first session, it was found that the best hypouricemic effect dosage of quercetin was 10mg/(kg·d). Dosage lower or higher than this level showed opposite effect. In the second part, the quercein content in the powder onion was 1066.74 mg/kg..In the last part, all of the three substrates showed remarkably hypouricemic effect by comparing with the model group had significant difference (P< 0.05).The activity levels of XOD in serum and liver were significantly decreased in the quercein and powder onion treated rats (P<0.05), while apigenin had no marked effect on the XOD level in rats (P>0.05). The activities of SOD and T-AOC in liver were significantly higher in the rats treated with quercetin and powder onion than that of apigenin treated rats (P<0.05).ConclusionQuercetin has hypouricemic effect at appropriate dosage level in hyperuricemic rats. Similarity, apigenin, and powder onion containing same dose of quercetin also could lower serum uric acid. The possible mechanisms of quercetin and powder onion are through reducing the activity of XOD and ADA, increasing antioxidant capacity and reducing lipid peroxidation. The hypouricemic effect of Apigenin might be through other means rather than affecting XOD activity. Quercetin and powder onion have advanced renal protecting effect in hyperuricemia rats than apigenin does, which might be due to their stronger antioxidant capacity. |
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