Effects Of Alcohol On The Expressions Of Neurotransmitters In Bone Marrow Mesenchymal Stem Cells Of Rabbit

Background and Objective:In recent years, drinking is a necessary part of life, the necrosis of femoral head cases became more and more because of alcoholism. But the precise etiopathogenesis of ONFH remains unclear. Pervious studies showed that there is BMSCs exist in the bone marrow which could differentiate into osteoblast, chondroblast, adipocyte or neurocyte. Studies have demonstrated that alcohol not only could induce the BMSCs differentiate into adipocyte,but also effect the expression of neurotransmitters, then the formation and reparation of bone tissue and blood vessel are inhibited, and the osteonecrosis developed finally. There was report about that the expressions of neurotransmitters are effected by alcohol in animal body. In this experiment, the BMSCs was isolated and cultured in vitro, we added alcohol in the culture fluid,the effects were observed with the method of molecular biology to investigate the mechanism of alcohol-induced osteonecrosis.Methods: The BMSCs was distilled from the bone marrow of the limbs which was cultivated and isolated by adherence after taking in operation. The 3rd dynasty BMSCs were divided into two groups at random. In the experimental group, the cells were gived 0.09mol/L alcohol. In the control group, the cells were treated without alcohol. The expressions of the neurotransmitters mRNA in the cells, which were treated with or without 0.09mol/L alcohol on day 4,7,11,15, was investigated by the reverse transcription-polymerase chain reaction (RT-PCR).In addition,the in culture fluid in cells was determined after the cells was intervened 14 days.Results:The expressions of NGFmRNA, FGFmRNA, CGRPRmRNA, VIPRmRNA and SPRmRNA in the experimental groups were significantly lower than that in the control groups, so do the content of OCN and the activity of ALP, but the expression of PPARymRNA increased drastically(.P<0.01). There were no statistically differences compared among the experimental groups(P>0.05).Conclusion:Alcohol could depress the expressions of the neurotransmitters or their receptors which could promote the processes of osteogenic differentiation in BMSCs, this might be one of the mechanisms for the development of the alcohol-induced osteonecrosis.