The Study Of Concentrated Growth Factor (CGF) Promoting BMSCs Osteogenesis

ObjectivesBone grafts are used to correct congenital bone defects,as well as bone defects caused by traumatic injuries or cancer.The most common problems affecting this type of surgery are bone absorption and donor trauma.In recent years,with the rapid development of tissue engineering and related technologies,the study of bone regeneration provides a new opportunity.Materials and MethodsCGF was prepared from rat heart blood;BMSCs were harvested from rat bilateral femurs.Apoptosis assay,live-dead assay and cell proliferation assay compared with cell viability cultivated with CGF or not.Alizarin Red staining and Von Kossa staining helped illustrate the effect of CGF on osteogenic differentiation and mineralization of BMSCs.The expression of osteogenic genes for BMSCs cultured in CGF extracts was detected by quantitative real-time PCR assay and western blot.In the vivo study,five repair groups were created for comparative purposes:(?)CGF+BMSCs;(?)CGF;(?)collagen+BMSCs;(?)collagen;(?)blank.After three months,the rats were sacrificed,and histopathology and three-dimensional CT images produced.ResultsConcentrated growth factor(CGF)promotes the in vitro osteogenic differentiation of bone marrow-derived stromal cells(BMSCs).It enhances the viability of BMSCs,suppresses apoptosis and promotes the expression of the OPN and Runx2 osteogenesis genes.ALP staining,Alizarin Red staining and Von Kossa staining demonstrate the differences between groups.Bone regeneration was significantly higher in the(?)CGF+BMSC group;osteogenesis was lower in the(?)CGF and(?)collagen+BMSC groups,at very similar levels;the(?)collagen and(?)blank groups scored the lowest results.ConclusionsThe platelet growth factors provide abundant osteoconductive growth factors.Our research suggests that combining CGF with BMSCs leads to the formation of fibrin scaffolds that have a powerful effect on osteogenesis as well as a subsidiary angiogenetic effect.The CGF+BMSC combination is a promising biological material for bone regeneration.