The Study Of The Relevance In TCM Syndrome Of Colorectal Cancer And ERCC1 Gene Polymorphisms

Objective:To investigate the association between ERCC1 (excision repair cross-complementation groupl) C8092A and C19007T Gene Polymorphisms and different TCM syndrome types of Colorectal Cancer, laying genetic foundation for the comprehensive study of colorectal TCM syndrome.Methods:The patients with Colorectal Cancer were collected between February 2010 and October 2010 in Medical Oncology, Oncological Surgery, Gastrointestinal Surgery of fujian provincial hospital, according to a double standard in Western medicine to tell their classification, type. The control group is healthy patients for the same period. Gene polymorphisms of ERCC1 C8092A and C19007T in each group were examined by polymorphisms chain reaction and sequencing. Statistical analysis.Results:1. The frequencies of C8092A genotype as CC and CA+AA in case group were 44.4% and 55.6%,in the control group were 70.0% and 30.0%, having high significance statistics difference(P< 0.05); The frequencies of C allele in case group and in the control group were 62.6% and 81.7%, The frequencies of A allele in case group and in the control group were 37.4% and 18.37%, difference having statistics significance (P<0.05)2. The frequencies of C19007T genotype as CC and CT+TT in case group were 51.5% and 48.5%,in the control group were 56.7% and 43.3%, having no significance statistics difference(P>0.05); The frequencies of C allele in case group and in the control group were 71.2% and 71.7%, The frequencies of T allele in case group and in the control group were 28.8% and 28.3% , no statistically significant diferences were found between two groups (P >0.05)3. The frequencies of C8092A genotype as CC and CA+AA in different TCM syndromes in case group have no significance statistics difference(P>0.05);And there were also no statistically significant differences in the frequencies of C allele or A allele among different TCM syndromes in case group(P>0.05). The frequencies of genotype,in Damp and hot accumulate knot type,Qi stagnancy and blood stasis syndrome and the control group, have no significance statistics difference (P>0.05); Statistically significant diferences were found in Spleen and kidney Yang type,Liver-kidney Yin deficiency type and the control group (P< 0.05). The frequencies of C allele or A allele, in Damp and hot accumulate knot type,Qi stagnancy and blood stasis syndrome and the control group, have no significance statistics difference (P>0.05); Statistically significant diferences were found in Spleen and kidney Yang type,Liver-kidney Yin deficiency type and the control group(P<0.05).4. The frequencies of C19007T genotype as CC and CT+TT in different TCM syndromes in case group have significance statistics difference (P<0.05); However, the frequencies,in Damp and hot accumulate knot type and Qi stagnancy and blood stasis syndrome, in Spleen and kidney Yang type and Liver-kidney Yin deficiency type, have no significance statistics difference(P>0.05); Statistically significant diferences were found in Damp and hot accumulate knot type and Spleen and kidney Yang type,Liver-kidney Yin deficiency type, also in Qi stagnancy and blood stasis syndrome and Spleen and kidney Yang type,Liver-kidney Yin deficiency type(P<0.05); The frequencies of C allele and T allele among different TCM syndromes in case group have significance statistics difference(P< 0.05); However,the frequencies, in Damp and hot accumulate knot type and Qi stagnancy and blood stasis syndrome, in Spleen and kidney Yang type and Liver-kidney Yin deficiency type, have no signif-icance statistics difference (P>0.05); Statistically significant diferences were found in Damp and hot accumulate knot type and Spleen and kidney Yang type,Liver-kidn-ey Yin deficiency type, also in Qi stagnancy and blood stasis syndrome and Spleen and kidney Yang type(P< 0.05);But, no statistically significant diferences were found between Qi stagnancy and blood stasis syndrome and Liver-kidney Yin deficiency type(P>0.05). The frequencies of genotype or allele have no statistically significant diferences between different TCM syndromes in case group and the control group(P>0.05).Conclusion:1.The C8092A variation of ERCC1 gene associated with the development of colorectal cancer, compared with control group, ERCC1 C8092A CA, AA type gene may be the predisposing gene of colorectal cancer; The C19007T variation of ERCC1 gene may not be associated with the development of colorectal cancer.2. ERCC1 C8092A Gene Polymorphisms may not be associated with different TCM syndrome types of Colorectal Cancer and may be associated with asthenia synd-syndrome of colorectal cancer.3.ERCC1 C19007T Gene Polymorphisms associated with sthenia syndrome and asthenia syndrome of colorectal cancer; ERCC1 C19007T CC type gene and C allele are correalted with asthenia syndrome of colorectal cancer; ERCC1 C19007T CT,TT type gene and T allele are correalted with sthenia syndrome of colorectal cancer.