| Background and ObjectiveCervical squamous cell carcinoma is the most common malignant tumor of the female reproductive system, more than half of malignant tumor; It is a serious threat to women's quality of health and life, according to the occurrence of cervical squamous cell carcinoma, the domestic and foreign scholars research mechanism is flourishing. From protein levels of research of cervical squamous cell carcinoma in the pathogenesis, clinical diagnosis, differential diagnosis and guiding the treatment, has the extremely vital significance.Twist in the experiment of fruit flies, it was first identified in a series of play an important role in growth process, especially in the initial stages of the mesoderm generation is particularly obvious. In 1996, was first cloning, Twist genes in human 7p21.2 sites, including two explicit the son, and a introns, coding 203 amino acids, molecular quality about 28kd. Alkaline spiral-ring-spiral family (basic Helix-Loop-bHLH) Helixt contained within, Twist genes and other genes, the same or heterogeneous bHLH gene fusion, two segments combined into DNA structure domain, with special style and E-box unifies, so can special hike or interference target genes transcription process.E-cadherin is a very important adhesion molecules, is a single across the membrane glycoprotein, maintain epithelial type. Each other between the molecules by exocellular immunoglobulin structure domain, and by establishing ties, beta serial protein and alpha actin cytoskeleton connection between cells, and then formed union. In most malignant tumors are found in the cadherin factor lack of E-between cells or the mutual adsorption function decline disappear, mutual sticky and cell decreases the capability of the malignant cells, and from the posteromedial separation down, transfer to other organs.Epithelial cells interstitial transformation (EMT) is embryonic development, chronic inflammatory reaction and tumor progression of a major stages, in most malignant tumor growth stage EMT occupy significant position. Its salient features are make epithelial cell polarity disappear and obtain interstitial characteristics of cancer cells, which have an important effect on metastasis. Experiment is bHLH family, as a member of cadherin Twist to the synthesis process of its influence, upstream produced and considering the transcription process of interference, make cancer further deterioration greater likelihood. In embryonic generation EMT plays an important role in the process, it can be used as a malignant tumor cell deterioration of starting. The transcription stage, in the role of E-box Twist, prompting sequence starting factor EMT process, cause E-cadherin generate interference.Study found that Twist is a new type of cancer genes, it can interfere with cell death, the physiological process involving P53 dependence and not rely on channel. Twist-siRNA can promote its function very lack of P53 breast cancer and melanoma for prostate cancer cell apoptosis, Twist the reduction in the number of study found that cells can be easier, it is because cancer cell apoptosis P53, concluded that their lack of cells can Twist P53 channel apoptosis adjusted.Now that the experimental progress in cancer, Twist down-regulate occurrence and development and deteriorating process is important, but it is still not clear the occurrence mechanism. In cervical squamous cell carcinoma in Twist over-active and drop in cancer progression and degeneration role without further experiment and discuss still. According to the above discussion, this study applies immunohistochemical method detection Twist, E-cadherin and P53 three related gene expression in each of the cervical tissue paper, Twist, E-cadherin and P53 abnormal cervical squamous cell carcinoma expression in the mutual relationship between the occurrence and development of three factors, and the correlation between expression, this paper analyzes Twist, E-cadherin and P53 protein expression and clinical parameters of the relationship, further cervical squamous cell carcinoma of the pathogenesis is expounded, the occurrence of cervical squamous cell carcinoma for development, provide scientific basis for invasion and metastasis.Material and MethodsCollect the second affiliated hospital of zhengzhou university department during December 2009 and April 2010 cervical tissue of 150 cases cervical squamous cell carcinoma, including 50 cases, cervical intraepithelial neoplasia of cervical intraepithelial neoplasia variable organization,50 patients (CINâ…¡22 cases CINâ…¢28 cases), chronic cervicitis organization 50 cases. Using immunohistochemistry (SP) detection method of cervical squamous cell carcinomas organization, who cervical intraepithelial neoplasia of variable organizations and chronic cervicitis organization Twist,E-cadherin and P53 protein expression, and the results SPSS11.0 statistics statistical application package treatment for inspection standards alpha=0.05.Results1. Twist,E-cadherin and P53 cervical tissue in different expressions positioning: immunohistochemical Twist protein displayed in the cytoplasm positive dyed; E cadherin protein displayed in the cell membrane and (or) cytoplasmic positive dyed; P53 protein show positive stain nuclei.2. Twist,E-cadherin and P53 in cervical tissue in different express strength: Twist and P53 protein in cervical squamous cell carcinoma in protein expression strength higher than chronic cervicitis and cervical intraepithelial neoplasia of variable; E-cadherin protein in the cervical squamous cell carcinoma in expression strength below chronic cervicitis and cervical intraepithelial neoplasia of change. 3. Twist,E-cadherin and P53 in cervical tissue in different expression rate quite: Twist protein expression level of cervical squamous cell carcinoma in than chronic cervicitis and cervical intraepithelial neoplasia of change, a statistically significant, and chronic cervicitis and cervical intraepithelial neoplasia of Twist the expression level variable group was not statistically significant. E-cadherin protein expression level of cervical squamous cell carcinoma in than chronic cervicitis and cervical intraepithelial neoplasia of change, a statistically significant, and chronic cervicitis and cervical intraepithelial neoplasia of variable group E-cadherin expression level was not statistically significant. P53 protein from chronic cervicitis, cervical intraepithelial neoplasia of cervical squamous cell carcinoma variable to expression level gradually increasing, with statistical significance.4. Twist,E-cadherin and P53 protein expression and clinical parameters: the relationship of Twist protein expression level in different clinical staging cervical squamous cell carcinoma, presence of lymph node metastasis and between different pathological differentiation degree compared with statistical significance. E-cadherin protein expression in different level of cervical squamous cell carcinoma in clinical stages and different pathological differentiation degree is compared between significant in lymph node metastasis, and positive and negative groups set no statistically significant difference between. P53 protein expression in different level of cervical squamous cell carcinoma in clinical stages and different pathological differentiation degree is compared between significant in lymph node metastasis, and positive and negative groups set no statistically significant difference between.5.in the cervical squamous cell carcinoma the correlation of the analysis between Twist protein expression and E-cadherin,P53:Twist protein expression and E-cadherin is negative correlation; Twist protein expression and P53 of positive correlation exists between.Conclusions1. Twist protein may participate in the occurrence of cervical squamous cell carcinoma, reflect the development process of cervical squamous cell carcinoma, and malign rate of the cervical squamous cell carcinoma has certain influence on invasion and metastasis. 2.E-cadherin protein may become clinical treatment and assessment is an important reference prognostic indicators.3.P53 protein in the pathogenesis of cervical squamous cell carcinoma plays an important role.4.Twist protein may also affect P53 and E-cadherin cervical squamous cell carcinoma, induce happening and transfer to invade. |
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