The Study On Propagation Technique Of The Rare Medical Plant-Anemone Flaccida Fr. Schmidt

The dried rhizome of Anemone flaccida Fr.Schmidt,which is also called Diwu,is the officinal part,it is of great valuable to be exploited and used,because its particular efficacy for detoxication and ease pain.A.flaccida mainly distributed alone the Yangtze River.Because it growes very slowly,and the ferine resource is very limited,it is essential to culture artificially to solve the problem of deficient resource in its exploiture.In this paper,some propagation work of A.flaccida was studied,including investigating its growth habitat,breaking the dormancy of seeds and rhizomes and tissue culture,for providing technique to protect the wild resources and develop artificial cultivation.The results were shown as follow:1.A.flaccida grew in shade of hillside,adapting to clammy and cold environment. The period of its growth was very short and it grew very slowly.It started growing in early March,flowered in early April,its florescence lasted about 10 days.It fruited in late April,and above-ground died gradually and the rhizome came into dormancy in late May.,The whole period of growth and development was less than 90 days.2.The seed viability was detected very low.Phytohormone(including GA₃,6-BA, ethephon),chemical reagent(including KNO₃,H₂O₂) and stratification had no effect on seed germination.3.Both low temperature(4℃～16℃) and phytohormones,including GA₃(12.5 mg/L～100 mg/L) and 6-BA(1 mg/L～20 mg/L) could break the dormancy of rhizome and induce to form lateral buds.The best treatment of sprouting speed for apical buds were 100 mg/L GA₃ and1 mg/L～20 mg/L 6-BA at 20℃;as diameter and length of apical bud growth for purpose,the best treatment were 20 mg/L 6-BA under 20℃and 20mg/L 6-BA under 8℃～16℃;as for forming density of lateral buds,the best treatment was 10 mg/L 6-BA under 20℃.At the same temperature,density of lateral buds of rhizome treated with 6-BA was larger than that treated with GA₃,when the consistence of 6-BA exceed 10 mg/L,the density of lateral buds descend with increasing the consistence of 6-BA and the optimal concentration of 6-BA was increasing as the increasing of temperature.Considering the speed and rate of germination and the size of apical buds,and the density and size of lateral buds,dipping rhizome with 20 mg/L 6-BA for about 24h at 20℃was recommended to break dormancy of buds and induce to form more lateral buds in produce.4.Carbohydrates deposit in rhizome translated in breaking dormancy.At first, starch as the main store substance,degraded into soluble sugar,which resulted in soluble sugar increased to meet the nutritional requirement for germination. 5.In tissue culture,it was difficult to induce the callus using leaves and rhizomes as explants in MS culture medium adding different phytohormones,contrarily,it could induce a little of callus from buds as explants,but that callus stopped growing and died gradually.It could obtain plantlet directly from germinating bud as explants in culture medium of MS,but there was no connection observed between the number of plantlet and category and concentration of phytohormones,perhaps,the number of plantlet was related to explants self.Otherwise,contamination of explants in tissue culture was related to the time and place for sampling,and categories of fungoid were various,so it was very difficult to control,which increased workload and difficulties.