The Genetic Diversity Of Three Types Of Lespedeza Hedysaroides (Pall.) Kitag

The research studied the genetic diversity from morphology, protein, chromosome and moleculeslevels in three types of Lespedeza hedysaroides (Pall.) Kitag.. Main results were as followed:1. Three types of Lespedeza hedysaroides were rich of morphological diversity. The ANOVAanalysis results showed that, plant height, basal stem diameter, leaf, seed, thick stem weight and middlestem weight et al. total19traits showed a significant difference in three types of Lespedezahedysaroides. Correlation analysis showed14pairs of traits were significantly positive correlation and16pairs of traits were significant negative correlation. The main indicators of the first principalcomponent, which were arrayed as effect degree including the plant height, sublevel branches, basestem diameter, footstalk length, primary branches, et al. total20indexs delegating70%of the totalinformation.2. Salt soluble proteins and seed storage proteins in the common-type and deep green-type ofLespedeza hedysaroides were different from the tall-type. The protein molecular weights ranged from12.43to154.53kDa.6bands were shared among the three types and13bands were specific andpolymorphism accounted for68.42%of total19salt soluble protein bands. In the total seed storageproteins were19bands shared and9bands were specific and polymorphism accounted here for32.14%of the total28bands.3. The number of chromosomes were the same among3types of Lespedeza hedysaroides, whichwas2n=2x=20, belonged to diploid. The karyotype formula was2n=2x=14m+4sm+2T (SAT) of thecommon-type Lespedeza hedysaroides, which was2B type; the karyotype asymmetric coefficient was60.63%. The deep green-type's karyotype formula was the same to the common-type, but was2A type,and the karyotype asymmetric coefficient was62.01%; the tall-type Lespedeza hedysaroides karyotypeformula was2n=2x=12m+4sm+4T (SAT), belonged to2B type, the karyotype asymmetric coefficientwas64.16%.4. Nine primers of ISSR were utilized and total109bands were obtained by amplication reaction,each primer showed average88.91%of polymorphism. The effective number of alleles was1.6996, theindex of Nei's genetic diversity was0.3908and the Shannon's information index was0.5601of threetypes of Lespedeza hedysaroides; ISSR clustering showed that the deep green-type and tall-type wereclassified as one group; the common-type was another. The dendrogram of phenotype and proteinsdiversity suggested that the common-type and deep green-type were clustered as one group, the tall-typewere classified as a different group. There were obvious differences among three types of Lespedezahedysaroides and the difference between the tall-type and the common-type Lespedeza hedysaroideswas significant.