White Spores In Micropropagation Of Key Technology Research

This study using the fern spores of Diplopterygium glaucum as the experimental material,the spore germination induction explant sterilization, selection of culture medium, plant growthregulators, sucrose concentration, temperature, type of light, pH value of solution and other factorson the spore propagation were studied in this text, this text is good to the rapid propagation andutilization of gametophytes, it plays an important role in the genera, divided fern origin, evolution,ecological distribution of Diplopterygium glaucum. The results are showed as follows:1．The results showed that2%NaClO and70%alcohol combination sterilizing are best.2%NaClO30min70%alcohol sterilization and sterilization,15s, the survival rate of85is best.2． The results are different when using different medium,1/2MS medium sporegermination rate is highest, reaching81.1%, germination time is the shortest, at the13D began togermination, is a better culture medium.3. Auxin and cytokinin on spore reproduction has certain effect. On the whole, all sorts ofauxin can promote the white spore germination,2,4-D were the best, formation of spore breedingtime is the shortest,7d, and spore propagation efficiency highest, up to70%. All kinds ofcytokinins, TDZ induction effect is best, spore germination time is the shortest, at a concentrationof2mg/L induce the highest rate, reached61.1%, only need about8D spores begin germination.All medium combinations, the best combination of medium was modified MS+2,4-D0.2mg/L+NAA0.2mg/L+TDZ3mg/L.4．Different concentrations of sucrose on white spore inducing effects of different inductionrate, spore as the sucrose concentration had different changes, when the sucrose concentration is30.0g/L, white spore inducing rate reaches a maximum of91.48%.5. When the light intensity of80μmol·m-2·s-1, spore germination rate is the highest,reaching92.17%. Spore is needed in light conditions the initiation ofgermination,20-50/μmol·m-2·s-1low intensity can induce the germination.6. Temperature also affects the white spore reproduction. The test results show that, at25degrees C spore germination in the shortest time required, is about7d.7. White spore propagation process, culture medium solution pH value5.6the highestgermination rate,83.2%white spore adorable The rate of no significant difference, but when pH value reaches7.6significantly reduced.