Transcriptomic Profiling Of Physiological Male Sterility Induced By Chemical Hybridizing Agent SQ-1in Wheat(Triticum Aestivum)

Heterosis is one of the critical ways to improve the yield of wheat. At present, heterosiswas studied mostly focus on the following field: cytoplasmic male sterility, male sterilityinduced by chemical hybridizing agent, photo-temperature sensitive male sterility. Andchemical hybridizing agent (CHA method) on wheat becomes an important and convenientway of utilizing the heterosis in wheat. A great amount of practice suggested that this methodcan be used on the basis of the latest advance in wheat seed producing. Chemical hybridizingagent SQ-1is one of the most excellent agents used on wheat. SQ-1performs great in makingmale sterility in common wheat and has very little interaction between different varieties.What's more, it has very little interference on the agricultural traits and fertility of the stigma,while very little negative impact was detected on the seeds. All this characters above makeSQ-1an excellent chemical hybridizing agent. However, the mechanism of male sterilityinduced by SQ-1is still out of our knowledge. In order to reveal how male sterility wasinduced in wheat by chemical hybridizing agent and to provide solid theoretical support onthe heterosis in wheat, gene chip was used for analyzing the profile of the gene expressed inShannong558after treated by chemical hybridizing agent SQ-1in this study. The aim of thisstudy was to lay a foundation for future explanation of the mechanism of the male sterilityinduced by chemical hybridizing agent. The main results obtained were as follows:(1) Gene chip was used for analyzing the profile of the gene expressed in Shannong558after treatment with chemical hybridizing agent SQ-1, and2052genes were detected to havechanged on expression level. In all the differentially genes,1294genes were up-regulated and758genes were down-regulated. Program Cluster3.0was also used to perform the clusteranalysis of all the signal strength of gene chip. (2) The differentially genes were analyzed GO Terms enrichment, and most up-regulatedgenes were classified into toxin metabolic process and response to abiotic stimulus whiledown-regulated genes were enriched in the pathway of synthesis of polysaccharide.(3) Semi-quantity PCR and Real Time PCR were carried out to verify the genes changedmost, it turns out that all the genes verified have similar expression pattern with the patternmeasured by gene chip, most importantly the correlation between them is very good.