Molecular Mechanism On Phsiological Male Sterile Wheat Induced By Gametocide SQ-1

Nowadays, Chemical hybrid agent (CHA) is an important and convenient way of heterosis。A lot of facts suggest that it can build hybrid breeding on the latest fruit of wheat conventional breeding。SQ-1 is a new type of wheat Gametocide which can lead to male complete sterility, and without any side effects on the pistil fertility and agronomic traits, However, its mechanism of inducing wheat male sterility is not clear yet. In order to reveal the molecular mechanism and provide theoretical and technical support for utilization of heterosis, nine candidate genes were selected from AFLP-cDNA library of Physiological male Sterile lines induced by gametocide SQ-1 and cloned and analyzed using semi-quantitative RT-PCR in this study. The results were as follows:1. wheat XiNong 2611 and XiNong 9766 induced by Chemical hybrid agent (CHA) SQ-1 and their near fertile lines were used as materials the expression pattem.of nine candidate genes were compared in sterile and fertile anthers. The results showed Enolase,Pyruvate dehydrogenase E1 subunit (E1-PDHc), Mitochondrial NAD7 gene in male sterile anthers, were inhibited; but Cytochrome C oxidase subunitⅦ, Pyrimidine nucleotide disulfide oxidoreductase and the Ascorbate peroxidase (APX), expressed highest ahead in sterile anthers Speculated that glycolysis, Krebs cycle and electron transport process was inhibited in anthers, so the energy shortage of anthers developvent leaded to the anther abortion in early microspore development that is, the early uninucleate stage.SQ-1 treatment in the physiological male sterile anthers ARF-GTP protein was inhibited membrane transport function may be blocked, causing material required for the normal development of microspores in short supply, there is a certain relationship with male sterility; the pan-Pathway related gene:ubiquitin-ribosomal protein S27a fusion and F-box protein, in the sterile anther highest abundance of gene expression in the period ahead, suggesting that the critical period in the development of small spores, sterile anthers large number of ubiquitin-related gene expression to accelerate the degradation of a useful protein is closely related with male sterility.2. Using the first strand cDNA Xinong 2611 as template, E1-PDHc gene (GenBank accession GU563379) and ARF-GTP gene (GenBank accession GU563378) was cloned..GU563379 sequence fragment length 1206bp, encoding 330 amino acids, with a typical plant El-PDHc gene conserved domain, and through domain analysis and homology alignment with the typical plant ARF-GTP gene conserved domain, and GU563378contains a complete ORF, ORF sequence was 1380bp in lengthencoding 460 amino acids.