Study On A Male Sterile Mutant TR1376A From Progeny Of A Transgenic Plant Containing P_SAG12-IPT In Wheat

The study on male sterility of wheat plays an important role on utilization of heterosis. Inorder to further explore wheat genetic mechanism of male sterility, iIn this paper, we studiedthe male sterile plant TR1376A from following aspects: fertility genetic, pollen abortion andpollen microscope structures, and molecular marker technology. This study used SSR andAFLP techniques to map the male sterility gene and construct the genetic linkage map ofmale-sterility wheat TR1376A. The results showed that:1. F1hybrids of male sterile plant TR1376A and9male male fertile varieties gave1:1segregation of male fertility to sterility, indicating that the male sterility of TR1376A washeritable and controlled by a single dominant gene, all infertility is heterozygous plantgenotype.2. The pollen activity was investigated by2,3,5-triphenyltetrazolium chloride (TTC)and IKI respectively. Compared with fertile pollen, infertility pollen was dyed less shallowly.Under the same magnification field view of microscope, the number of infertility pollen isless, which contrasted with fertile poolen.The data indicated that infertility pollen number wasfewer, and most of them were pooly developed or aborted.3. Using fertile and sterile DNA bulks to screen1049SSR primers, the result showed thatthere existed polymorphism in10primers. They were Xbarc129, Xbarc232, Xbarc380,Xgdm98, Xcfd13, Xcfd29, Xcfd46, Xcfd76, Xcfd188and Xwmc28. Subsequently, using apopulation of170individuals to verify the primers that showed polymorphism,3SSR primers,Xgdm98, Xcfd188and Xcdf76showed stable polymorphism and all located in chromosome6D of wheat.4. Using fertile and sterile DNA bulks to screen512AFLP primer combinations, theresult showed that there existed polymorphism in30primers. Then, a small population andusing a population of170individuals to verify the primers that showed polymorphism,3primers, P-GCA/M-GAC, P-GTT/M-GTG, P-TGT/M-GGG,showed stable polymorphism.But the genetic distance between primer combinations P-GCA/M-GAC, P-TGT/M-GGG andthe target gene were larger than40cM, so that only primer combination P-GTT/M-GTG was closely linked to target gene.5. Using MapDraw V2.1drew genetic linkage map, the genetic distance betweenmicrosatellite locis, Xgdm98, Xcfd188, Xcfd76and target gene were19.2cM,7.7cM,9.5cMrespectively. They were distributed on both sides of target gene. AFLP markers locatedbetween SSR markers and target gene, the distance between AFLP markers and target genewas4.7cM. The identification of the four markers will lay the foundation for the isolationand cloning sterile gene of wheat.